New England Journal of Medicine August 31, 2017

Alectinib versus Crizotinib in Untreated ALK-Positive Non-Small-Cell Lung Cancer

Peters S, Camidge DR, Shaw AT, et al. (ALEX Trial Investigators)

Bottom Line

In patients with previously untreated, advanced ALK-positive NSCLC, first-line treatment with alectinib resulted in significantly longer progression-free survival and dramatically lower rates of central nervous system progression compared to crizotinib.

Key Findings

1. Alectinib significantly improved investigator-assessed progression-free survival compared to crizotinib (HR 0.47; 95% CI, 0.34 to 0.65; P<0.001) [2.1.1].
2. The 12-month event-free survival rate was 68.4% with alectinib versus 48.7% with crizotinib.
3. Median progression-free survival was not reached in the alectinib group at the time of primary analysis, compared to 11.1 months in the crizotinib group.
4. Alectinib dramatically reduced the risk of central nervous system (CNS) progression compared to crizotinib, with a cause-specific hazard ratio of 0.16 (95% CI, 0.10 to 0.28; P<0.001).
5. CNS progression events occurred in only 12% of patients treated with alectinib (18 of 152) compared to 45% of those treated with crizotinib (68 of 151).
6. The objective response rate was numerically higher with alectinib (82.9%) compared to crizotinib (75.5%), though the difference was not statistically significant (P=0.09).
7. Grade 3 to 5 adverse events were less frequent in the alectinib arm (41%) than in the crizotinib arm (50%), demonstrating a superior safety profile despite patients having a longer median duration of treatment on alectinib.

Study Design

Design
Randomized Controlled Trial
Open-Label
Sample
303
Patients
Duration
18.6 mo
Median
Setting
Global multicenter
Population Patients with previously untreated, advanced ALK-positive non-small-cell lung cancer (NSCLC), including those with asymptomatic central nervous system (CNS) disease
Intervention Alectinib 600 mg orally twice daily
Comparator Crizotinib 250 mg orally twice daily
Outcome Investigator-assessed progression-free survival (PFS)

Study Limitations

The open-label design could theoretically introduce assessment bias for the primary endpoint, although this was mitigated by an independent review committee (IRC) that confirmed the investigator-assessed progression-free survival findings.
Overall survival (OS) data were immature at the time of the primary publication, limiting initial conclusions about long-term survival advantages.
The allowance of crossover following progression on crizotinib confounds subsequent long-term overall survival analyses.

Clinical Significance

The ALEX trial catalyzed a paradigm shift in the management of advanced ALK-rearranged NSCLC. By demonstrating a 53% reduction in the risk of disease progression and an 84% reduction in the risk of CNS progression compared to the first-generation inhibitor crizotinib, alectinib established highly-selective, brain-penetrant second-generation ALK inhibitors as the unequivocal standard of care in the first-line setting.

Historical Context

Following the PROFILE 1014 trial, the first-generation ALK/ROS1/MET inhibitor crizotinib became the standard first-line targeted therapy for ALK-positive NSCLC. However, resistance almost inevitably developed within a year, most problematically presenting as isolated central nervous system (CNS) metastases due to crizotinib's poor blood-brain barrier penetration. Alectinib was designed as a highly selective second-generation ALK inhibitor with robust CNS penetrance. Following the Japanese J-ALEX trial, which evaluated a lower dose of alectinib (300 mg BID), the global ALEX trial decisively confirmed the superiority of the standard global dose (600 mg BID) over crizotinib in the primary treatment setting.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

What is the pharmacological mechanism that explains why alectinib has a dramatically lower rate of central nervous system (CNS) progression compared to crizotinib?

Key Response

Crizotinib is a substrate for P-glycoprotein (P-gp), a major efflux pump located at the blood-brain barrier, which actively pumps the drug out and limits its CNS penetration. Alectinib is not a substrate for P-gp, allowing it to readily cross the blood-brain barrier, achieve high concentrations in the cerebrospinal fluid, and effectively treat or prevent CNS metastases, which are very common in ALK-positive NSCLC.

Resident
Resident

A newly diagnosed patient with metastatic ALK-positive NSCLC presents with asymptomatic brain metastases. Based on the ALEX trial, how does this finding impact your initial choice of systemic therapy versus local CNS therapy?

Key Response

Historically, upfront whole-brain radiation or stereotactic radiosurgery was the standard of care for brain metastases. The ALEX trial demonstrated that alectinib has excellent CNS penetration and a high CNS objective response rate. Therefore, for asymptomatic brain metastases in ALK+ NSCLC, upfront treatment with alectinib is preferred, often allowing patients to safely delay or completely avoid the neurocognitive toxicities associated with brain irradiation.

Fellow
Fellow

With alectinib establishing itself as the first-line standard in ALK-positive NSCLC, how does the landscape of acquired resistance mutations shift compared to the post-crizotinib setting, and what are the implications for next-line targeted therapy?

Key Response

Resistance to first-generation crizotinib is often mediated by the ALK L1196M 'gatekeeper' mutation, which second-generation inhibitors like alectinib easily overcome. However, progression on upfront alectinib frequently involves more recalcitrant mutations, most notably ALK G1202R, or off-target bypass tracks (e.g., MET amplification). This necessitates sequencing to a third-generation TKI like lorlatinib, which was specifically rationally designed to overcome the G1202R mutation, rather than utilizing another second-generation TKI.

Attending
Attending

The ALEX trial showed a profound PFS benefit but immature Overall Survival (OS) data at the time of initial publication. In clinical practice, how do you justify the paradigm shift to upfront alectinib over a sequencing strategy (crizotinib followed by alectinib at progression) in the absence of mature OS data?

Key Response

While OS is the traditional gold standard, the dramatic reduction in CNS progression with upfront alectinib profoundly protects quality of life and cognitive function, avoiding the immediate morbidity of CNS disease and radiation. Furthermore, alectinib has a markedly better overall tolerability profile (less GI toxicity, less hepatotoxicity, and fewer visual disturbances) than crizotinib. The 'save the best for last' sequencing argument fails here because CNS progression on crizotinib can cause irreversible neurological deficits or death before a patient ever reaches second-line therapy.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

The trial utilized a cause-specific hazard model and cumulative incidence functions to analyze time to CNS progression. Why is treating systemic progression or death as a competing risk methodologically superior to standard Kaplan-Meier estimation for this specific CNS endpoint?

Key Response

If standard Kaplan-Meier methodology were used, patients dying or progressing systemically would be censored. This violates the assumption of independent censoring, because death definitively precludes the possibility of future CNS progression. Using a competing risks framework (such as the Fine-Gray subdistribution hazard model) provides a mathematically unbiased and clinically accurate estimate of the actual probability of CNS progression in a population where systemic failure or death frequently occurs first.

Journal Editor
Journal Editor

Given that the control arm utilized crizotinib at a time when its limitations regarding CNS penetration were already well-documented, how does the lack of an alternative next-generation ALK inhibitor control arm impact the critical appraisal of the ALEX trial's superiority claims?

Key Response

A seasoned peer reviewer would flag that comparing a highly CNS-penetrant second-generation TKI to a poorly penetrant first-generation TKI almost guarantees a statistical win on CNS-specific endpoints. While crizotinib was the universally accepted standard of care at the trial's inception, by the time of publication, the field was already utilizing ceritinib and brigatinib. The editorial significance remains exceptionally high due to the sheer magnitude of the PFS benefit and favorable toxicity profile, but the 'weak comparator' for CNS endpoints is a standard methodological limitation that must be contextualized.

Guideline Committee
Guideline Committee

Based on the ALEX trial's findings regarding PFS, CNS efficacy, and toxicity, how should clinical practice guidelines (e.g., NCCN, ASCO) reclassify the level of evidence and preference for first-line therapies in advanced ALK-positive NSCLC, particularly regarding the historical use of crizotinib?

Key Response

The ALEX trial provides definitive Category 1 evidence for alectinib as a preferred first-line therapy. Consequently, guideline committees must demote crizotinib from a 'preferred' status. Current NCCN guidelines reflect this by listing alectinib (along with brigatinib and lorlatinib) as 'Preferred' first-line options, while crizotinib is relegated to 'Other Recommended' or reserved for regions where next-generation TKIs are unavailable, citing alectinib's unequivocally superior PFS, significantly lower CNS progression rate, and better toxicity profile.

Clinical Landscape

Noteworthy Related Trials

2014

PROFILE 1014

n = 343 · NEJM

Tested

Crizotinib 250mg twice daily

Population

Previously untreated advanced ALK-positive NSCLC

Comparator

Platinum-based chemotherapy

Endpoint

Progression-free survival

Key result: Crizotinib significantly prolonged progression-free survival compared to chemotherapy (10.9 vs 7.0 months) and increased objective response rates.
2017

J-ALEX Trial

n = 207 · Lancet

Tested

Alectinib 300mg twice daily

Population

Japanese patients with advanced ALK-positive NSCLC

Comparator

Crizotinib 250mg twice daily

Endpoint

Progression-free survival

Key result: Alectinib demonstrated superior progression-free survival and a favorable safety profile compared to crizotinib.
2020

CROWN Trial

n = 296 · NEJM

Tested

Lorlatinib 100mg once daily

Population

Previously untreated advanced ALK-positive NSCLC

Comparator

Crizotinib 250mg twice daily

Endpoint

Progression-free survival

Key result: Lorlatinib resulted in significantly longer progression-free survival and higher frequency of intracranial responses than crizotinib.

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