The Lancet SEPTEMBER 11, 2023

NALIRIFOX versus nab-paclitaxel and gemcitabine in treatment-naive patients with metastatic pancreatic ductal adenocarcinoma (NAPOLI 3): a randomised, open-label, phase 3 trial

Zev A. Wainberg, Davide Melisi, et al.

Bottom Line

The NAPOLI-3 phase 3 trial demonstrated that the NALIRIFOX regimen significantly improved overall and progression-free survival compared to the standard-of-care nab-paclitaxel plus gemcitabine in treatment-naive metastatic pancreatic ductal adenocarcinoma.

Key Findings

1. The primary endpoint was met, with NALIRIFOX demonstrating a superior median overall survival (OS) of 11.1 months compared to 9.2 months for the nab-paclitaxel plus gemcitabine arm (hazard ratio 0.83; 95% CI 0.70–0.99; P=0.036).
2. Median progression-free survival (PFS) was significantly improved in the NALIRIFOX group at 7.4 months versus 5.6 months in the control group (hazard ratio 0.69; 95% CI 0.59–0.84; P<0.0001).
3. Grade 3 or higher treatment-emergent adverse events were frequent in both arms, occurring in 87% of the NALIRIFOX group and 86% of the nab-paclitaxel plus gemcitabine group, with distinct toxicity profiles (higher rates of diarrhea and nausea in the NALIRIFOX arm, vs. higher rates of neutropenia and anemia in the nab-paclitaxel plus gemcitabine arm).
4. Treatment-related deaths were low and comparable, reported at 2% in the NALIRIFOX arm and 2% in the control arm.

Study Design

Design
RCT
Open-Label
Sample
770
Patients
Duration
16.1 mo
Median
Setting
Multicenter, 18 countries
Population Adult patients with previously untreated, histologically confirmed metastatic pancreatic ductal adenocarcinoma and an ECOG performance status of 0 or 1.
Intervention NALIRIFOX (liposomal irinotecan 50 mg/m2, 5-fluorouracil 2400 mg/m2, leucovorin 400 mg/m2, and oxaliplatin 60 mg/m2) on days 1 and 15 of a 28-day cycle.
Comparator Nab-paclitaxel (125 mg/m2) and gemcitabine (1000 mg/m2) on days 1, 8, and 15 of a 28-day cycle.
Outcome Overall survival in the intention-to-treat population.

Study Limitations

The trial was open-label, which introduces potential bias in the management of adverse events and assessment of secondary endpoints.
The study did not compare NALIRIFOX directly against the FOLFIRINOX regimen, which remains another established standard-of-care first-line treatment for metastatic pancreatic cancer.
The inclusion of unselected patients may obscure potential benefits in specific molecular or genomic subgroups that were not stratified.
The clinical meaningfulness of the modest OS gain (1.9 months) must be weighed against the increased toxicity burden associated with the NALIRIFOX regimen.

Clinical Significance

NAPOLI-3 established NALIRIFOX as a new, effective first-line treatment option for patients with metastatic pancreatic ductal adenocarcinoma. The results provide clinicians with an alternative to nab-paclitaxel/gemcitabine, particularly for patients who can tolerate a triplet chemotherapy regimen, although the choice between NALIRIFOX and FOLFIRINOX remains a topic for future comparative investigation.

Historical Context

Metastatic pancreatic ductal adenocarcinoma has historically had a poor prognosis, with limited therapeutic options. For over a decade, gemcitabine-based regimens and the FOLFIRINOX triplet have been the cornerstones of treatment. Liposomal irinotecan was previously approved only in the second-line setting after failure of gemcitabine-based therapy; NAPOLI-3 successfully moved this agent into the first-line setting by incorporating it into a modified, more tolerable triplet regimen.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

What is the pharmacological rationale for utilizing a liposomal formulation of irinotecan (nal-IRI) in the NALIRIFOX regimen compared to conventional free irinotecan?

Key Response

Liposomal irinotecan is designed to remain in the systemic circulation longer than free irinotecan. The liposomal bilayer protects the active drug from premature conversion to its active metabolite SN-38 by plasma carboxylesterases. This allows for preferential accumulation within tumor tissue via the enhanced permeability and retention (EPR) effect, where tumor-associated macrophages eventually break down the liposome, releasing the drug locally and theoretically increasing the therapeutic index.

Resident
Resident

Based on the safety data from NAPOLI-3, which specific adverse events should a clinician monitor more closely in a patient receiving NALIRIFOX compared to those receiving gemcitabine plus nab-paclitaxel?

Key Response

While both regimens are intensive, NALIRIFOX is associated with a higher incidence of gastrointestinal toxicities, specifically Grade 3/4 diarrhea (20% vs 16%) and nausea. Conversely, gemcitabine plus nab-paclitaxel typically shows higher rates of hematologic toxicity (such as Grade 3/4 anemia and neutropenia) and peripheral neuropathy. Clinicians must prioritize hydration and aggressive anti-diarrheal management for NALIRIFOX patients.

Fellow
Fellow

NAPOLI-3 demonstrated a median Overall Survival (OS) of 11.1 months for NALIRIFOX. How does this result compare to the historical data from the PRODIGE 4/ACCORD 11 trial, and why might NALIRIFOX be considered an alternative to modified FOLFIRINOX despite the lack of a direct head-to-head comparison?

Key Response

The OS of 11.1 months in NAPOLI-3 is remarkably similar to the 11.1 months reported for FOLFIRINOX in the landmark PRODIGE 4 trial. NALIRIFOX utilizes a lower dose of 5-FU and replaces conventional irinotecan with the liposomal form, potentially offering a more standardized, evidence-based triplet regimen for first-line use, especially since NAPOLI-3 was a global, multicenter phase 3 trial that included a broader patient population than the original FOLFIRINOX studies.

Attending
Attending

Given the success of NALIRIFOX in the first-line setting, how does this shift our sequencing strategy for metastatic pancreatic ductal adenocarcinoma, specifically regarding the use of 5-FU-based vs. Gemcitabine-based therapies in the second line?

Key Response

The movement of liposomal irinotecan (previously standard 2L after Gem-failure via NAPOLI-1) into the 1L setting creates a therapeutic vacuum in the 2L. If NALIRIFOX is used first, the most logical 2L option becomes Gemcitabine plus Nab-paclitaxel. This study essentially establishes two validated sequences (NALIRIFOX followed by Gem/Abraxane, or Gem/Abraxane followed by 5-FU/nal-IRI/LV), though the optimal sequence for total survival remains to be determined through real-world evidence.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

Critique the choice of the control arm in NAPOLI-3. Why was gemcitabine plus nab-paclitaxel selected instead of modified FOLFIRINOX, and how does this choice impact the statistical and clinical generalizability of the findings?

Key Response

Gemcitabine plus nab-paclitaxel was chosen as it is a globally accepted standard of care with level 1 evidence. Using FOLFIRINOX as a control would have required a much larger sample size to show superiority between two triplets. While this design successfully proves NALIRIFOX is superior to the doublet, it leaves unanswered whether the liposomal formulation provides a true benefit over the much less expensive conventional FOLFIRINOX triplet, potentially limiting uptake in cost-sensitive healthcare systems.

Journal Editor
Journal Editor

As a reviewer, how would you evaluate the impact of the open-label design on the secondary endpoints of Progression-Free Survival (PFS) and Objective Response Rate (ORR), and what measures in the trial design mitigate potential investigator bias?

Key Response

In open-label trials, investigator assessment of disease progression can be influenced by knowledge of the treatment arm, potentially inflating PFS. To mitigate this, NAPOLI-3 used standardized RECIST v1.1 criteria and mandated objective imaging. However, a 'tough' reviewer would look for Blinded Independent Central Review (BICR) results to validate that the 1.8-month PFS benefit was not skewed by earlier discontinuation or later assessment in the control vs. experimental arms.

Guideline Committee
Guideline Committee

Considering current ASCO and NCCN guidelines prioritize FOLFIRINOX or Gem/Abraxane for patients with ECOG PS 0-1, does NAPOLI-3 provide sufficient evidence to list NALIRIFOX as a 'Preferred' Category 1 recommendation, and should it be recommended over standard FOLFIRINOX?

Key Response

NAPOLI-3 provides high-level (Phase 3) evidence for OS and PFS improvement, warranting a Category 1 recommendation. Current guidelines (NCCN) already updated to include NALIRIFOX as a first-line option. However, without a head-to-head vs. standard FOLFIRINOX, it is unlikely to be recommended 'over' FOLFIRINOX; instead, it serves as a contemporary, standardized triplet option. The committee must weigh the incremental benefit against the significantly higher cost of liposomal irinotecan compared to generic irinotecan.

Clinical Landscape

Noteworthy Related Trials

2011

PRODIGE 4/ACCORD 11 Trial

n = 342 · NEJM

Tested

FOLFIRINOX

Population

Patients with metastatic pancreatic adenocarcinoma

Comparator

Gemcitabine monotherapy

Endpoint

Overall survival

Key result: FOLFIRINOX was associated with significantly longer overall survival than gemcitabine, though with increased toxicity.
2013

MPACT Trial

n = 861 · NEJM

Tested

Nab-paclitaxel plus gemcitabine

Population

Patients with metastatic pancreatic adenocarcinoma

Comparator

Gemcitabine monotherapy

Endpoint

Overall survival

Key result: Nab-paclitaxel plus gemcitabine demonstrated a statistically significant improvement in overall survival compared to gemcitabine alone.
2016

NAPOLI-1 Trial

n = 417 · Lancet

Tested

Nanoliposomal irinotecan (nal-IRI) plus fluorouracil and folinic acid

Population

Patients with metastatic pancreatic cancer previously treated with gemcitabine-based therapy

Comparator

Fluorouracil and folinic acid

Endpoint

Overall survival

Key result: The addition of nal-IRI to fluorouracil and folinic acid improved overall survival in patients previously treated with gemcitabine.

Tailored to your role

Want this tailored to you?

Add your specialty or training stage to get role-specific takeaways and more questions.

Personalize this analysis