The New England Journal of Medicine JANUARY 27, 2022

Balanced Multielectrolyte Solution versus Saline in Critically Ill Adults (The PLUS Trial)

The PLUS Investigators and the Australian and New Zealand Intensive Care Society Clinical Trials Group

Bottom Line

In a large, multicenter, randomized trial of critically ill adults, the use of a balanced multielectrolyte solution (Plasma-Lyte 148) compared to 0.9% saline did not result in a significant difference in 90-day all-cause mortality.

Key Findings

1. The primary outcome of 90-day all-cause mortality occurred in 21.8% of patients in the Plasma-Lyte 148 group and 22.0% in the saline group (absolute difference, -0.15 percentage points; 95% CI, -3.60 to 3.30; P=0.90).
2. There was no statistically significant difference between groups regarding the secondary outcomes of new renal replacement therapy, receipt of vasoactive drugs, or duration of mechanical ventilation.
3. Patients receiving Plasma-Lyte 148 exhibited slightly higher arterial blood pH and lower serum chloride levels during the first seven days, though these biochemical differences were not associated with improved clinical outcomes.

Study Design

Design
RCT
Double-Blind
Sample
5,034
Patients
Duration
90 days
Median
Setting
Multicenter, Australia/New Zealand
Population Critically ill adults admitted to the ICU who required fluid resuscitation and were expected to remain in the ICU for at least the next 3 days.
Intervention Plasma-Lyte 148 for all fluid resuscitation and crystalloid maintenance therapy while in the ICU.
Comparator 0.9% saline for all fluid resuscitation and crystalloid maintenance therapy while in the ICU.
Outcome All-cause mortality within 90 days after randomization.

Study Limitations

The study was conducted in a specific geographic region (Australia and New Zealand), which may limit the generalizability to other healthcare systems with different fluid resuscitation practices.
The trial was unblinded in terms of clinician preference for fluid choice in certain clinical scenarios, leading to some protocol deviations where patients received non-assigned fluids.
Despite the large sample size, the study may still be underpowered to detect very small, yet potentially clinically meaningful, differences in mortality rates.

Clinical Significance

The study suggests that for critically ill adults in the ICU, the choice between balanced multielectrolyte solutions like Plasma-Lyte 148 and 0.9% saline does not significantly impact 90-day mortality. Clinicians may choose between these fluids based on other factors, such as cost, availability, and specific patient electrolyte or acid-base requirements, rather than an expectation of mortality benefit from balanced solutions.

Historical Context

The choice of intravenous crystalloid for fluid resuscitation has been a subject of intense debate in critical care for decades. Historically, 0.9% saline was the standard, but concerns regarding its high chloride content and potential for hyperchloremic metabolic acidosis led to the investigation of 'balanced' crystalloids. Previous observational and cluster-randomized studies (such as SMART and SALT-ED) had suggested potential benefits for balanced solutions, prompting this large-scale, definitive randomized controlled trial to clarify the mortality impact.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

What is the physiological basis for the hypothesis that balanced multielectrolyte solutions, like Plasma-Lyte 148, might be superior to 0.9% saline in critically ill patients?

Key Response

0.9% saline is 'unbalanced' because its chloride concentration (154 mmol/L) is significantly higher than human plasma (approx. 100 mmol/L). Infusing large volumes of saline can induce hyperchloremic metabolic acidosis. Theoretically, this acidosis leads to renal afferent arteriole vasoconstriction and decreased glomerular filtration rate (GFR). Balanced solutions use organic anions like acetate or gluconate to replace some chloride, maintaining a more physiological pH and electrolyte balance.

Resident
Resident

The PLUS trial showed no difference in 90-day mortality between saline and balanced crystalloids. In light of this, how should a clinician approach fluid selection for a patient with severe traumatic brain injury (TBI) compared to a patient with septic shock?

Key Response

For most ICU patients (like those in septic shock), the choice between saline and balanced solutions appears to have negligible impact on mortality. However, in TBI, saline is often preferred because balanced solutions like Plasma-Lyte 148 are slightly hypotonic (effective osmolarity ~270 mOsm/L). Hypotonic fluids can promote cerebral edema and increase intracranial pressure. Clinical guidelines and subgroup analyses from fluid trials generally favor the use of isotonic 0.9% saline in neuro-critical care.

Fellow
Fellow

How does the achieved 'separation' in chloride levels and pH between the intervention and control groups in the PLUS trial influence your interpretation of its neutral findings compared to the earlier SMART trial?

Key Response

In the PLUS trial, the mean difference in plasma chloride between groups was only about 2 mmol/L. This limited separation suggests that either the 'toxic' threshold of chloride was not reached in the saline group or that the volume of fluid administered (median 3.9L) was insufficient to manifest the harm seen in observational studies or the SMART trial. If the dose of the 'intervention' (chloride avoidance) is low, detecting a mortality difference becomes statistically challenging, emphasizing that fluid type may only matter at very high volumes.

Attending
Attending

Given the neutral results of PLUS and the earlier BASICS trial, what are the primary teaching points regarding 'fluid stewardship' beyond simply the composition of the crystalloid?

Key Response

The focus of teaching should shift from the saline vs. balanced debate toward the 'Four Ds' of fluid therapy: Drug, Dose, Duration, and De-escalation. The PLUS trial suggests that for the average ICU patient, the *type* of crystalloid is less important than the *volume* administered. Clinicians should be taught to avoid fluid overload, utilize dynamic predictors of fluid responsiveness, and promptly transition to a conservative fluid strategy once hemodynamic stability is achieved.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

Critique the use of a 90-day mortality endpoint in the PLUS trial versus the 'Major Adverse Kidney Events within 30 days' (MAKE30) composite endpoint used in the SMART trial.

Key Response

Mortality is a 'hard' endpoint but is influenced by many factors beyond fluid choice, potentially requiring enormous sample sizes to show a difference. MAKE30 is a composite including mortality, new RRT, and persistent renal dysfunction. While composites increase statistical power and capture organ-specific harm (like chloride-induced AKI), they can be driven by their less clinically significant components (e.g., small creatinine rises). The choice between them reflects a trade-off between the clinical relevance of the outcome and the statistical feasibility of the study.

Journal Editor
Journal Editor

The PLUS trial utilized a pragmatic, double-blind design. What are the potential threats to internal validity introduced by 'pre-randomization' fluid exposure in this study population?

Key Response

Most patients in the PLUS trial received significant volumes of fluid (often saline) in the Emergency Department or during initial resuscitation before being randomized in the ICU. This 'contamination' means both groups had a baseline exposure to the control. If the harm of saline occurs early (the 'first hit'), the trial might fail to show a benefit of balanced solutions because the damage was already done, potentially leading to a type II error.

Guideline Committee
Guideline Committee

Based on the results of the PLUS trial and the 2021 Surviving Sepsis Campaign recommendations, should the preference for balanced crystalloids be upgraded from a 'weak' to a 'strong' recommendation?

Key Response

No. The Surviving Sepsis Campaign currently provides a 'weak recommendation, low quality of evidence' for balanced crystalloids. Because PLUS (and BASICS) were large, high-quality RCTs that failed to demonstrate a mortality benefit, they actually reinforce the idea that the benefit is marginal at best. The recommendation is likely to remain 'weak' or be downgraded to a statement of equivalence, acknowledging that while balanced fluids are more physiological, they have not proven to be life-saving compared to saline.

Clinical Landscape

Noteworthy Related Trials

2015

SALT Trial

n = 923 · JAMA

Tested

Balanced crystalloids

Population

Hospitalized adults receiving IV fluids

Comparator

Saline

Endpoint

Major adverse kidney events within 30 days (MAKE30)

Key result: Balanced crystalloids resulted in a lower incidence of MAKE30 compared to saline among hospitalized adults.
2018

SMART Trial

n = 15,802 · NEJM

Tested

Balanced crystalloids

Population

Critically ill adults in the ICU

Comparator

Saline

Endpoint

Major adverse kidney events within 30 days (MAKE30)

Key result: The use of balanced crystalloids reduced the risk of MAKE30 compared to saline among critically ill adults.
2021

BaSICS Trial

n = 10,520 · JAMA

Tested

Balanced crystalloids

Population

Critically ill adults

Comparator

Saline

Endpoint

90-day mortality

Key result: There was no statistically significant difference in 90-day mortality between balanced crystalloids and saline.

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