New England Journal of Medicine JUNE 01, 2023

Dostarlimab for Primary Advanced or Recurrent Endometrial Cancer (RUBY Trial)

Mirza MR, Chase DM, Slomovitz BM, et al.

Bottom Line

In patients with primary advanced or recurrent endometrial cancer, the addition of the PD-1 inhibitor dostarlimab to standard-of-care carboplatin-paclitaxel chemotherapy significantly improves both progression-free and overall survival compared to chemotherapy alone.

Key Findings

1. In the overall population, dostarlimab plus chemotherapy reduced the risk of death by 31% compared to chemotherapy alone (HR 0.69; 95% CI 0.54-0.89; P=0.0020).
2. The 24-month progression-free survival rate in the overall population was 36.1% in the dostarlimab group versus 18.1% in the placebo group (HR 0.64; 95% CI 0.51-0.80).
3. Patients with mismatch repair-deficient (dMMR) tumors experienced a profound survival benefit, with a 68% reduction in the risk of death (HR 0.32; 95% CI 0.17-0.63).
4. The safety profile was consistent with known side effects of immunotherapy and chemotherapy, with a higher incidence of immune-related adverse events in the dostarlimab group, though generally manageable.

Study Design

Design
RCT
Double-Blind
Sample
494
Patients
Duration
36 mo
Median
Setting
Multicenter, global
Population Adult patients with histologically or cytologically confirmed primary advanced (FIGO stage III or IV) or first recurrent endometrial cancer not amenable to curative surgery or radiation.
Intervention Dostarlimab (500 mg) plus carboplatin and paclitaxel every 3 weeks for 6 cycles, followed by dostarlimab monotherapy (1000 mg) every 6 weeks for up to 3 years.
Comparator Placebo plus carboplatin and paclitaxel every 3 weeks for 6 cycles, followed by placebo monotherapy every 6 weeks.
Outcome Progression-free survival (PFS) in the overall population and the dMMR/MSI-H population, and overall survival (OS) in the overall population.

Study Limitations

The trial reflects a diverse patient population including those with aggressive histologies, which may affect generalizability to specific rare subtypes.
While statistically significant, the absolute survival gain in the mismatch repair-proficient/microsatellite stable (pMMR/MSS) subgroup is more modest than in the dMMR population, necessitating careful patient selection.
Long-term toxicity data, specifically regarding rare, delayed immune-related adverse events, continue to be monitored as the trial matures.

Clinical Significance

The RUBY trial establishes a new frontline standard-of-care for patients with advanced or recurrent endometrial cancer, marking the first time an immune-checkpoint inhibitor combined with chemotherapy has demonstrated a clear overall survival benefit in this population.

Historical Context

For decades, platinum-based chemotherapy (carboplatin and paclitaxel) served as the primary systemic treatment for advanced endometrial cancer, with limited options available following disease progression. The RUBY trial represents a paradigm shift by integrating PD-1 blockade into the frontline setting, building on previous success with single-agent checkpoint inhibitors in later lines of therapy.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

How does a deficiency in DNA mismatch repair (dMMR) lead to an increased sensitivity to PD-1 inhibitors like dostarlimab in endometrial cancer?

Key Response

dMMR leads to the accumulation of numerous mutations (high tumor mutational burden), which results in the production of neoantigens. These neoantigens make the tumor more 'visible' to the immune system. Dostarlimab blocks the PD-1 receptor on T-cells, preventing the tumor from using the PD-L1 pathway to evade immune detection, thereby allowing the sensitized T-cells to attack the neoantigen-rich cancer cells.

Resident
Resident

In a patient with newly diagnosed Stage IV endometrial cancer that is mismatch repair proficient (pMMR), how do the RUBY trial results influence your decision to add dostarlimab to standard carboplatin-paclitaxel?

Key Response

The RUBY trial demonstrated a statistically significant improvement in progression-free survival (PFS) for the overall population, including the pMMR subgroup (HR 0.76). While the benefit is most dramatic in dMMR patients (HR 0.28), the resident must recognize that adding dostarlimab is now a valid consideration for pMMR patients, though they should also weigh the benefit against potential immune-related adverse events and alternative regimens like pembrolizumab/lenvatinib.

Fellow
Fellow

The RUBY trial utilized a dual primary endpoint of PFS in the dMMR/MSI-H population and PFS in the overall population. How does this hierarchical testing strategy impact the interpretation of the results for the pMMR cohort?

Key Response

By using a hierarchical or 'gatekeeping' strategy, the study ensures that the statistical significance in the overall population (which includes pMMR) is only formally tested if the dMMR population reaches significance. This protects the type I error rate. For a fellow, understanding that the overall population's success was driven largely by the dMMR effect size is crucial when discussing the relative magnitude of benefit in the pMMR subgroup.

Attending
Attending

Given the survival benefit seen in RUBY, how should the timing of molecular testing for MMR/MSI status be optimized in clinical practice to ensure appropriate first-line therapy?

Key Response

The RUBY results shift immunotherapy from the second-line (recurrent) setting to the first-line (primary advanced) setting. Therefore, universal MMR testing via IHC or NGS should be performed at the time of initial diagnosis or first biopsy of advanced disease, rather than waiting for recurrence, to ensure that the window for maximum benefit from dostarlimab plus chemotherapy is not missed.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

What are the limitations of using the RECIST v1.1 criteria to evaluate progression-free survival in a trial combining cytotoxic chemotherapy with a PD-1 inhibitor, and how might 'pseudoprogression' affect the RUBY trial's primary endpoint analysis?

Key Response

RECIST v1.1 may classify early immune-cell infiltration into the tumor as 'progression' (pseudoprogression), potentially underestimating the true clinical benefit of immunotherapy. While cytotoxic chemotherapy usually induces rapid shrinkage, the immune component may cause transient flares. Researchers often use iRECIST in exploratory analyses to account for this, ensuring that patients are not prematurely removed from a potentially beneficial therapy.

Journal Editor
Journal Editor

While the RUBY trial shows clear efficacy, what concerns regarding the 'standard of care' control arm might a reviewer raise given the concurrent development of other checkpoint inhibitor combinations like NRG-GY018?

Key Response

An editor would look for whether the control arm (carboplatin-paclitaxel alone) remained the true global standard throughout the trial's duration and how the RUBY data compares to the nearly simultaneous NRG-GY018 (pembrolizumab) results. They would flag the need for a discussion on cross-trial comparisons and whether the trial sufficiently addressed PD-L1 expression levels as a predictive biomarker versus MMR status alone.

Guideline Committee
Guideline Committee

Should the RUBY trial data prompt a change in the NCCN Guidelines to make dostarlimab plus carboplatin-paclitaxel a 'Preferred' Category 1 recommendation for dMMR primary advanced endometrial cancer?

Key Response

Yes. Prior to RUBY, the standard was chemotherapy alone, with immunotherapy reserved for second-line. Given the hazard ratio for PFS of 0.28 and an early signal of improved overall survival in the dMMR group, the evidence meets the criteria for Category 1. The committee must decide if the benefit in the pMMR group (HR 0.76) is strong enough to warrant a similar 'Preferred' status or if it should be a 'Specified Circumstance' recommendation.

Clinical Landscape

Noteworthy Related Trials

2019

GOG-258 Trial

n = 736 · NEJM

Tested

Chemotherapy plus radiotherapy

Population

Stage III or IV endometrial cancer

Comparator

Chemotherapy alone

Endpoint

Recurrence-free survival

Key result: The addition of radiotherapy to chemotherapy did not significantly improve recurrence-free survival compared to chemotherapy alone in advanced endometrial cancer.
2020

KEYNOTE-158 Trial

n = 90 · JCO

Tested

Pembrolizumab monotherapy

Population

Previously treated mismatch repair-deficient endometrial cancer

Comparator

None (Single-arm)

Endpoint

Objective response rate

Key result: Pembrolizumab showed durable antitumor activity in patients with previously treated mismatch repair-deficient advanced endometrial cancer.
2023

NRG-GY018 Trial

n = 816 · NEJM

Tested

Pembrolizumab plus chemotherapy

Population

Advanced or recurrent endometrial cancer

Comparator

Placebo plus chemotherapy

Endpoint

Progression-free survival

Key result: Pembrolizumab plus chemotherapy significantly improved progression-free survival compared to placebo plus chemotherapy, regardless of mismatch repair status.

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