The New England Journal of Medicine JULY 07, 2022

Trastuzumab Deruxtecan in Previously Treated HER2-Low Advanced Breast Cancer

Modi S, Jacot W, Yamashita T, et al.

Bottom Line

The DESTINY-Breast04 trial established trastuzumab deruxtecan as a new standard of care for patients with HER2-low metastatic breast cancer, demonstrating superior progression-free and overall survival compared to standard chemotherapy.

Key Findings

1. In the hormone receptor-positive cohort, trastuzumab deruxtecan significantly improved median progression-free survival (PFS) to 10.1 months compared to 5.4 months with physician's choice chemotherapy (hazard ratio 0.51; 95% CI 0.40–0.64; P<0.001).
2. Overall survival (OS) in the hormone receptor-positive cohort was significantly prolonged with trastuzumab deruxtecan (median 23.9 months) versus chemotherapy (median 17.5 months; hazard ratio 0.64; 95% CI 0.49–0.84; P=0.003).
3. Across the entire study population (including both hormone receptor-positive and hormone receptor-negative patients), median PFS was 9.9 months for trastuzumab deruxtecan versus 5.1 months for physician's choice (hazard ratio 0.50; 95% CI 0.40–0.63; P<0.001).
4. Median OS for the overall study population was 23.4 months with trastuzumab deruxtecan versus 16.8 months with chemotherapy (hazard ratio 0.64; 95% CI 0.49–0.84; P=0.001).
5. Common grade 3 or higher treatment-emergent adverse events included neutropenia (13.7% with T-DXd vs 26.4% with TPC) and anemia (8.1% with T-DXd vs 5.7% with TPC), with adjudicated drug-related interstitial lung disease or pneumonitis occurring in 12.1% of patients in the T-DXd arm.

Study Design

Design
RCT
Open-Label
Sample
557
Patients
Duration
18.4 mo
Median
Setting
Multicenter, international
Population Patients with unresectable and/or metastatic HER2-low (IHC 1+ or IHC 2+/ISH-) breast cancer previously treated with 1 or 2 lines of chemotherapy in the metastatic setting.
Intervention Trastuzumab deruxtecan (5.4 mg/kg intravenously every 3 weeks).
Comparator Physician's choice of chemotherapy (eribulin, capecitabine, gemcitabine, nab-paclitaxel, or paclitaxel).
Outcome Progression-free survival in patients with hormone receptor-positive disease, assessed by blinded independent central review.

Study Limitations

The trial was open-label, which introduces potential bias in the assessment of subjective endpoints and symptom management.
The study excluded patients with previously untreated brain metastases, a common concern in clinical management of metastatic breast cancer.
The risk of interstitial lung disease/pneumonitis requires careful monitoring, which may affect its use in clinical practice.
The study focused on patients who had received 1–2 lines of prior chemotherapy, leaving questions regarding the efficacy of T-DXd in earlier or later lines of therapy.

Clinical Significance

DESTINY-Breast04 is a landmark trial that redefined the HER2-low population as a distinct, actionable category for HER2-targeted therapy. By demonstrating that an antibody-drug conjugate (trastuzumab deruxtecan) is superior to standard physician's choice chemotherapy in patients with HER2-low metastatic disease, the trial has fundamentally shifted the treatment paradigm for a large subset of breast cancer patients who were previously classified as HER2-negative.

Historical Context

Historically, HER2 status was strictly classified as either 'positive' (requiring HER2-directed therapy) or 'negative' (not requiring HER2-targeted therapy). The 'HER2-low' category, representing tumors with IHC 1+ or IHC 2+/ISH- expression, was excluded from HER2-targeted approaches. This trial provided the definitive evidence required to move beyond binary HER2 categorization, validating the use of specialized antibody-drug conjugates to exploit lower levels of target protein expression.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

What is the unique 'bystander effect' mechanism of Trastuzumab Deruxtecan (T-DXd), and why does it allow this drug to be effective in HER2-low tumors while earlier agents like T-DM1 were not?

Key Response

Unlike T-DM1, T-DXd utilizes a highly membrane-permeable topoisomerase I inhibitor payload and a high drug-to-antibody ratio. When the drug is internalized and the payload is released in a HER2-expressing cell, the payload can cross the cell membrane to kill adjacent tumor cells regardless of their HER2 expression levels. This is critical in HER2-low cancers where HER2 expression is often heterogeneous.

Resident
Resident

In the management of a patient starting T-DXd based on the DESTINY-Breast04 protocol, which specific adverse event requires the most vigilant monitoring due to its potential for high-grade toxicity, and what are the standard management steps if it is suspected?

Key Response

Interstitial Lung Disease (ILD)/pneumonitis is the most significant toxicity (occurring in 12.1% of patients in the trial). Management involves proactive monitoring for cough, dyspnea, or fever, regular CT scans, and immediate discontinuation of the drug for even Grade 1 symptoms, typically followed by systemic corticosteroid administration.

Fellow
Fellow

The DESTINY-Breast04 trial included a small cohort of patients with hormone receptor (HR)-negative, HER2-low disease. How do these results influence the treatment sequencing for patients traditionally classified as 'Triple-Negative' Breast Cancer (TNBC)?

Key Response

The trial showed a significant PFS and OS benefit even in the HR-negative cohort (PFS 8.5 vs 2.9 months). This effectively redefines a subset of TNBC as HER2-low, placing T-DXd as a preferred later-line option, potentially competing with or following Sacituzumab Govitecan in the treatment algorithm for these patients.

Attending
Attending

With the establishment of HER2-low as a clinically actionable therapeutic category, how does this study challenge our current reliance on the binary IHC classification system (0 vs. 1+), and what are the implications for patients previously labeled as IHC 0?

Key Response

The study creates a therapeutic 'gold rush' for IHC 1+ and 2+ results, but IHC was originally optimized to identify high expression (3+), not to distinguish 0 from 1+. This highlights the need for more sensitive assays (like quantitative proteomics or digital imaging) to identify 'HER2-ultralow' patients who might still benefit from the bystander effect but are currently excluded based on outdated binary scoring.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

Critique the use of 'Physician's Choice' as the control arm in DESTINY-Breast04. How might the heterogeneity of the control treatments (capecitabine, eribulin, gemcitabine, etc.) affect the interpretation of the hazard ratios for progression-free survival?

Key Response

While 'Physician's Choice' reflects real-world clinical practice and increases the trial's external validity, it introduces variability in the control arm's efficacy. If the distribution of selected chemotherapies was imbalanced or if certain agents were less effective in the HER2-low population, it could potentially inflate the perceived benefit of T-DXd compared to a single, more potent comparator.

Journal Editor
Journal Editor

As a reviewer, what concerns would you raise regarding the reproducibility of the 'HER2-low' designation given the documented 30-50% inter-pathologist discordance in distinguishing IHC 0 from IHC 1+?

Key Response

The primary threat to the study's validity is the lack of a standardized, highly reproducible assay for the lower end of the HER2 spectrum. If the 'HER2-low' status is not reliably reproducible across different labs, the generalizability of the trial's efficacy to the broader clinical population is compromised, as many 'IHC 0' patients might actually be 'IHC 1+' and vice-versa.

Guideline Committee
Guideline Committee

Based on the OS benefit demonstrated in DESTINY-Breast04, how should the NCCN and ASCO guidelines be updated for HR-positive metastatic breast cancer that has progressed on endocrine therapy and at least one line of chemotherapy?

Key Response

Current guidelines have been updated to include T-DXd as a Category 1 recommendation for HER2-low patients in the second-line or later setting. The strength of the OS data (17.5 months vs 13.9 months in the HR+ cohort) justifies moving T-DXd ahead of standard single-agent chemotherapies (like eribulin or capecitabine) for patients who meet the IHC 1+ or 2+/ISH-negative criteria.

Clinical Landscape

Noteworthy Related Trials

2012

EMILIA Trial

n = 991 · NEJM

Tested

Trastuzumab emtansine (T-DM1)

Population

HER2-positive advanced breast cancer patients previously treated with trastuzumab and a taxane

Comparator

Capecitabine plus lapatinib

Endpoint

Progression-free survival and overall survival

Key result: T-DM1 showed significant improvements in both progression-free and overall survival compared to standard chemotherapy regimens.
2019

NSABP B-47 Trial

n = 3270 · Lancet Oncol

Tested

Adjuvant trastuzumab plus chemotherapy

Population

HER2-low (IHC 1+ or 2+/FISH negative) early breast cancer

Comparator

Chemotherapy alone

Endpoint

Invasive disease-free survival

Key result: The addition of trastuzumab to standard adjuvant chemotherapy did not improve invasive disease-free survival in patients with HER2-low breast cancer.
2022

DESTINY-Breast03 Trial

n = 524 · NEJM

Tested

Trastuzumab deruxtecan

Population

HER2-positive metastatic breast cancer patients previously treated with trastuzumab and a taxane

Comparator

Trastuzumab emtansine (T-DM1)

Endpoint

Progression-free survival

Key result: Trastuzumab deruxtecan significantly improved progression-free survival compared with T-DM1.

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