New England Journal of Medicine AUGUST 29, 2017

Effects of Anacetrapib in Patients with Atherosclerotic Vascular Disease

The HPS3/TIMI55-REVEAL Collaborative Group (Bowman L, Hopewell JC, et al.)

Bottom Line

In patients with established atherosclerotic vascular disease already receiving intensive statin therapy, the addition of the CETP inhibitor anacetrapib led to a modest but significant reduction in major coronary events over a median follow-up of 4.1 years.

Key Findings

1. Anacetrapib significantly reduced the primary composite endpoint of major coronary events (coronary death, myocardial infarction, or coronary revascularization) to 10.8% compared to 11.8% in the placebo group (rate ratio 0.91; 95% CI 0.85–0.97; P=0.004).
2. Lipid-modifying effects were profound, with anacetrapib increasing HDL cholesterol by 104% (43 mg/dL) and reducing non-HDL cholesterol by 18% (17 mg/dL) relative to placebo at the study midpoint.
3. The clinical benefit of anacetrapib appeared to emerge after at least 3 years of treatment.
4. There were no significant safety concerns, specifically no increased risk of cancer or major non-vascular mortality, though minor increases in blood pressure and small reductions in kidney function were observed.

Study Design

Design
RCT
Double-Blind
Sample
30,449
Patients
Duration
4.1 yr
Median
Setting
Multicenter, international
Population Patients aged ≥50 years with established atherosclerotic vascular disease (history of myocardial infarction, cerebrovascular disease, peripheral artery disease, or diabetes with symptomatic coronary heart disease) receiving intensive atorvastatin therapy.
Intervention Anacetrapib 100 mg daily
Comparator Matching placebo daily
Outcome First major coronary event, defined as the composite of coronary death, myocardial infarction, or coronary revascularization.

Study Limitations

The clinical benefit observed was modest (9% relative risk reduction) despite the very large increase in HDL cholesterol, confirming that HDL elevation per se is not the primary driver of cardiovascular protection.
The benefit required prolonged treatment (≥3 years) to become statistically apparent, limiting the short-term clinical utility.
Despite the successful trial outcome, the drug was not brought to market due to the perceived clinical benefit not justifying the development costs in the era of potent, low-cost statins and other newer lipid-lowering therapies.

Clinical Significance

The trial successfully demonstrated that CETP inhibition can reduce cardiovascular events in high-risk patients, definitively shifting the narrative from previous CETP inhibitor trials that showed either harm (torcetrapib) or futility (dalcetrapib, evacetrapib); however, it also highlighted the diminished marginal clinical benefit of further non-LDL lowering strategies in the context of already intensive statin therapy.

Historical Context

The CETP inhibitor class had a troubled history, with multiple high-profile phase III failures: torcetrapib was terminated for increased mortality, while dalcetrapib and evacetrapib were stopped for lack of efficacy. REVEAL was the largest and longest trial for the class, providing the first clear signal of efficacy for a CETP inhibitor, though it ultimately did not lead to clinical adoption.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

How does the mechanism of action of anacetrapib differ from that of statins, and what does the failure of previous CETP inhibitors suggest about the 'HDL-C hypothesis'?

Key Response

Statins work by inhibiting HMG-CoA reductase, leading to an upregulation of LDL receptors. Anacetrapib inhibits Cholesterol Ester Transfer Protein (CETP), which normally moves cholesterol from HDL to LDL/VLDL. While this significantly raises HDL-C and lowers LDL-C, previous failures (like torcetrapib) showed that simply raising HDL-C level is insufficient to reduce cardiovascular risk if the particles are not functional or if there are off-target effects like hypertension.

Resident
Resident

In the REVEAL trial, anacetrapib reduced major coronary events by 9% in patients already on intensive statin therapy. How does this absolute risk reduction influence your decision to add a second-line agent compared to the evidence for ezetimibe or PCSK9 inhibitors?

Key Response

The REVEAL trial showed a modest absolute risk reduction (10.8% vs 11.8%). Residents should recognize that while statistically significant, the effect size is smaller than that seen in the FOURIER (evolocumab) or IMPROVE-IT (ezetimibe) trials. Clinical management often prioritizes agents with larger effect sizes, better safety profiles, or more favorable pharmacokinetics over anacetrapib, which accumulates in adipose tissue.

Fellow
Fellow

Anacetrapib exhibits a significant delay in the divergence of the Kaplan-Meier curves for cardiovascular events. How does its unique pharmacokinetic profile—specifically its lipophilicity and adipose tissue accumulation—complicate the assessment of its long-term safety and efficacy?

Key Response

Anacetrapib has a half-life of several years due to its storage in body fat. This means the drug continues to exert a lipid-lowering effect long after the last dose is taken. For a fellow, this implies that the 'true' efficacy might be underestimated in a 4-year trial, but it also creates a significant 'legacy' safety risk where adverse events cannot be quickly mitigated by drug discontinuation.

Attending
Attending

The REVEAL trial was a 'success' statistically, yet the manufacturer decided not to seek regulatory approval. What does this paradox teach us about the threshold for 'clinically meaningful' benefit in modern preventive cardiology?

Key Response

This is a key teaching point: statistical significance (p < 0.001) does not always translate to clinical or commercial viability. In an era of highly effective, generic statins and potent PCSK9 inhibitors, a drug that offers only a 9% relative risk reduction with a complicated PK profile may not provide enough incremental value to justify its cost and potential long-term risks.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

The REVEAL trial utilized a median follow-up of 4.1 years. Given the cumulative nature of atherosclerotic risk reduction, what statistical challenges arise when analyzing a drug whose therapeutic effect is predicated on the 'cholesterol-years' hypothesis rather than an immediate stabilization of plaque?

Key Response

The PhD-level challenge is the 'lag to benefit.' Traditional Cox proportional hazards models assume proportional effects over time, but CETP inhibitors often show non-proportionality as the benefit grows the longer the LDL/non-HDL cholesterol is suppressed. This requires specialized landmark analyses or time-dependent covariates to accurately model the increasing treatment effect over the study duration.

Journal Editor
Journal Editor

As a reviewer, how would you critically appraise the use of a 'run-in period' in the REVEAL trial, and to what extent does this design choice threaten the external validity of the findings regarding drug tolerability?

Key Response

The REVEAL trial included a 12-week run-in period where only those who tolerated the drug and statin were randomized. While this increases the power to detect an effect by ensuring compliance, it can mask the true incidence of side effects in the general population (selection bias). A tough reviewer would flag that the reported safety profile is only applicable to the 'selected' population, not the 'intent-to-treat' population in real-world practice.

Guideline Committee
Guideline Committee

Given the results of REVEAL, should CETP inhibitors be added to the AHA/ACC Multi-Society Guideline on the Management of Blood Cholesterol for secondary prevention in patients with persistent hypercholesterolemia?

Key Response

The committee would likely decline to include anacetrapib. Current guidelines (e.g., 2018 AHA/ACC) prioritize Ezetimibe and PCSK9 inhibitors (Class I and IIa) for very high-risk patients because their benefit-to-risk ratio is better established. Since anacetrapib is not available for clinical use and its benefit is modest compared to existing non-statin therapies, it does not currently meet the criteria for a recommendation in standard clinical pathways.

Clinical Landscape

Noteworthy Related Trials

2007

ILLUMINATE Trial

n = 15,067 · NEJM

Tested

Torcetrapib

Population

Patients at high risk for cardiovascular events

Comparator

Placebo

Endpoint

Composite of CV death, MI, stroke, or unstable angina

Key result: Torcetrapib increased HDL cholesterol but was terminated early due to an increase in mortality and cardiovascular events.
2012

dal-OUTCOMES Trial

n = 15,871 · JAMA

Tested

Dalcetrapib

Population

Patients with recent acute coronary syndrome

Comparator

Placebo

Endpoint

Time to first occurrence of a composite of CV death, MI, stroke, or unstable angina

Key result: The trial was terminated for futility as there was no clinical benefit despite the lipid-modifying effects of the drug.
2016

ACCELERATE Trial

n = 12,092 · JAMA

Tested

Evacetrapib

Population

Patients with high-risk vascular disease

Comparator

Placebo

Endpoint

Time to first occurrence of a major adverse cardiovascular event

Key result: Evacetrapib significantly increased HDL cholesterol and decreased LDL cholesterol but failed to show a reduction in cardiovascular outcomes.

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