The Lancet August 18, 2012

Mepolizumab for severe eosinophilic asthma (DREAM): a multicentre, double-blind, placebo-controlled trial

Ian D Pavord, Stephanie Korn, Peter Howarth, Eugene R Bleecker, Roland Buhl, Oliver N Keene, Hector Ortega, Pascal Chanez

Bottom Line

In patients with severe eosinophilic asthma, intravenous mepolizumab significantly reduced the rate of clinically significant asthma exacerbations compared to placebo, establishing the clinical efficacy of IL-5 inhibition for this specific phenotype.

Key Findings

1. Mepolizumab significantly reduced the rate of clinically significant exacerbations compared to placebo across all tested intravenous doses: 75 mg (1.24 vs 2.40 exacerbations/year; 48% reduction, p<0.0001), 250 mg (1.46 vs 2.40/year; 39% reduction, p=0.0005), and 750 mg (1.15 vs 2.40/year; 52% reduction, p<0.0001).
2. There were no significant improvements in secondary outcome measures of daily asthma control, including pre-bronchodilator FEV1, Asthma Control Questionnaire (ACQ) scores, or Asthma Quality of Life Questionnaire (AQLQ) scores compared to placebo.
3. The safety profile was comparable to placebo, with approximately 80% of patients experiencing adverse events across all groups; the most common adverse events were headache and nasopharyngitis.

Study Design

Design
RCT
Double-Blind
Sample
621
Patients
Duration
52 wk
Median
Setting
13 countries
Population Patients aged 12-74 years with a clinical diagnosis of asthma, a history of recurrent severe asthma exacerbations (≥2 in the previous year requiring systemic corticosteroids), and objective signs of eosinophilic airway inflammation (sputum eosinophils ≥3%, exhaled nitric oxide ≥50 ppb, peripheral blood eosinophils ≥300 cells/mcL, or rapid deterioration of asthma control after ≤25% reduction in steroids).
Intervention Intravenous mepolizumab at doses of 75 mg, 250 mg, or 750 mg administered every 4 weeks for 13 infusions.
Comparator Matched intravenous placebo (100 mL 0.9% NaCl) administered every 4 weeks for 13 infusions.
Outcome Rate of clinically significant asthma exacerbations, defined as validated episodes of acute asthma requiring treatment with oral corticosteroids, hospital admission, or an emergency department visit.

Study Limitations

The trial exclusively evaluated intravenous formulations of mepolizumab (75 mg, 250 mg, 750 mg), whereas the eventual FDA-approved and clinically utilized regimen is 100 mg administered subcutaneously.
Mepolizumab failed to demonstrate significant improvements in lung function (FEV1) or patient-reported daily symptom scores (ACQ, AQLQ) in this study, though these were observed in later trials like MENSA.
The 52-week follow-up period limited the assessment of long-term immunogenicity and prolonged safety of the biologic.

Clinical Significance

The DREAM trial was a landmark proof-of-concept and dose-ranging study that validated IL-5 as a critical therapeutic target for severe eosinophilic asthma. By demonstrating that mepolizumab halved the rate of severe exacerbations in patients with biomarker-proven eosinophilic inflammation, it shifted the paradigm of asthma management toward precision medicine. Although it did not show FEV1 or symptom improvements in this cohort, it successfully laid the clinical foundation for the approval and widespread use of anti-IL-5 biologics, sparing many patients from the morbidities of long-term systemic corticosteroids.

Historical Context

Historically, severe asthma was treated as a monolithic condition using escalating doses of inhaled and systemic corticosteroids. Early trials of anti-IL-5 therapies in unselected asthma populations failed to demonstrate clinical benefit. The DREAM trial resurrected anti-IL-5 therapy by utilizing a biomarker-driven approach, restricting enrollment specifically to patients with evidence of eosinophilic airway inflammation and a history of recurrent exacerbations. This successfully established the eosinophilic asthma endotype as a highly treatable trait.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

Mepolizumab specifically targets Interleukin-5 (IL-5). What is the physiological role of IL-5 in the immune system, and why is targeting it highly effective in the specific phenotype of asthma studied in the DREAM trial?

Key Response

IL-5 is the primary cytokine produced by Th2 cells and Type 2 innate lymphoid cells (ILC2s) that is responsible for eosinophil production, maturation, activation, and survival in the bone marrow and tissues. By blocking IL-5, mepolizumab depletes circulating and airway eosinophils, directly halting the inflammatory cascade that drives exacerbations in eosinophilic asthma.

Resident
Resident

The DREAM trial selected patients with severe eosinophilic asthma. In your clinical practice, how do you define and identify this specific phenotype, and what practical biomarkers should be checked before initiating a biologic like mepolizumab?

Key Response

Eosinophilic asthma is characterized by an elevated absolute eosinophil count (typically >150-300 cells/mcL), elevated fractional exhaled nitric oxide (FeNO), and/or sputum eosinophils, usually in patients with severe exacerbations despite high-dose ICS/LABA therapy. In practice, a standard CBC with differential to check the absolute eosinophil count is the most accessible and critical initial biomarker to justify IL-5 inhibitor therapy.

Fellow
Fellow

The DREAM trial demonstrated a significant reduction in exacerbations, but notably, it did not show a clinically significant improvement in FEV1 or asthma daily symptom scores. How do you reconcile this disconnect pathophysiologically, and how does this affect your counseling when starting a patient on mepolizumab?

Key Response

IL-5 inhibition effectively prevents the acute eosinophilic spikes that drive severe acute exacerbations. However, it may not reverse fixed airway remodeling, smooth muscle hypertrophy, or non-eosinophilic pathways that drive daily symptoms or baseline bronchoconstriction. Patients must be counseled that the primary goal of mepolizumab is to prevent severe attacks and reduce oral corticosteroid burden, not necessarily to serve as a quick fix for daily shortness of breath.

Attending
Attending

The DREAM trial utilized intravenous mepolizumab, yet current clinical practice almost exclusively uses the subcutaneous formulation. How does the foundational data from the DREAM trial integrate with later studies (like MENSA) to support this transition, and what are the practice-management implications of using SC formulations?

Key Response

DREAM established the clinical efficacy and safety profile of IV mepolizumab across multiple doses, revealing a plateau in the dose-response curve that suggested lower doses could be effective. This laid the groundwork for the MENSA trial, which validated the 100 mg SC dose. For attendings, the shift to SC administration radically altered practice by enabling home administration, improving patient compliance and access, but requiring a shift in how clinicians monitor long-term adherence outside the infusion center.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

The DREAM trial used a dose-ranging design (75 mg, 250 mg, and 750 mg IV) compared to placebo. From a methodological standpoint, how does splitting the active cohort into three dosage arms impact the statistical power for detecting rare adverse events, and what do the plateauing results suggest about the target's pharmacodynamics?

Key Response

Allocating a fixed overall sample size across multiple active arms reduces the N per arm, thereby decreasing the statistical power to detect rare, dose-dependent adverse events (inflating the risk of a Type II error for safety signals). The lack of superior clinical efficacy at 750 mg versus 75 mg suggests that systemic IL-5 receptor saturation, or maximal achievable eosinophil depletion, occurs at much lower systemic concentrations, which successfully guided subsequent lower-dose pharmaceutical development.

Journal Editor
Journal Editor

As a peer reviewer evaluating the DREAM trial, one might heavily scrutinize the inclusion criteria, which relied partly on historical exacerbation rates and fluctuating sputum eosinophils. How does the episodic nature of asthma introduce the risk of 'regression to the mean' in this trial, and how effectively does the placebo arm mitigate this threat to internal validity?

Key Response

Because asthma severity fluctuates, patients often enroll in trials following a peak in symptoms or exacerbations. Their subsequent exacerbation rates naturally decline, which is a classic 'regression to the mean'. While a robust, randomized, double-blind placebo arm theoretically controls for this because both groups should regress equally, a reviewer must verify that baseline peak eosinophil counts and historical exacerbations were perfectly balanced; any subtle asymmetry could artificially inflate the perceived treatment effect size.

Guideline Committee
Guideline Committee

Based on the evidence generated by the DREAM trial and subsequent supporting trials, how do current GINA (Global Initiative for Asthma) guidelines classify the strength of recommendation and level of evidence for mepolizumab, and where exactly does it fit in the stepwise treatment algorithm?

Key Response

GINA guidelines incorporate mepolizumab at Step 5. They provide a strong recommendation with Level A evidence for the use of anti-IL-5 biologics as add-on therapy in patients with severe, uncontrolled Type 2 (eosinophilic) asthma despite optimized high-dose ICS/LABA. The DREAM trial was instrumental in establishing this high level of evidence by demonstrating a definitive reduction in the rate of clinically significant exacerbations in this meticulously phenotyped population.

Clinical Landscape

Noteworthy Related Trials

2014

MENSA Trial

n = 576 · NEJM

Tested

Mepolizumab 75mg IV or 100mg SC every 4 weeks

Population

Patients with severe eosinophilic asthma and frequent exacerbations

Comparator

Placebo

Endpoint

Rate of clinically significant asthma exacerbations

Key result: Mepolizumab significantly reduced the rate of asthma exacerbations by roughly half and improved asthma control compared to placebo.
2016

SIROCCO Trial

n = 1204 · Lancet

Tested

Benralizumab 30mg SC every 4 or 8 weeks

Population

Patients with severe uncontrolled asthma and elevated blood eosinophils

Comparator

Placebo

Endpoint

Annual asthma exacerbation rate

Key result: Benralizumab significantly reduced the annual rate of asthma exacerbations and improved lung function in patients with severe eosinophilic asthma.
2018

LIBERTY ASTHMA QUEST Trial

n = 1902 · NEJM

Tested

Dupilumab 200mg or 300mg SC every 2 weeks

Population

Patients with uncontrolled moderate-to-severe asthma

Comparator

Placebo

Endpoint

Annualized rate of severe asthma exacerbations

Key result: Dupilumab resulted in significantly lower rates of severe asthma exacerbations and better lung function than placebo, particularly in patients with elevated eosinophils.

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