New England Journal of Medicine JULY 24, 2024

Twice-Yearly Lenacapavir or Daily F/TAF for HIV Prevention in Cisgender Women: Interim Analysis Results from the PURPOSE 1 Study

Linda-Gail Bekker, et al. for the PURPOSE 1 Study Team

Bottom Line

In this Phase 3 trial, subcutaneous lenacapavir administered twice-yearly demonstrated 100% efficacy in preventing HIV-1 infection among adolescent girls and young women in sub-Saharan Africa compared to a background HIV incidence rate.

Key Findings

1. Lenacapavir achieved 100% efficacy in preventing HIV-1 acquisition, with zero incident infections observed among the 2,138 participants in the lenacapavir group (incidence rate 0 per 100 person-years) compared to a calculated background HIV incidence of 2.41 per 100 person-years.
2. The incidence of HIV-1 infection in the lenacapavir group was significantly lower than that observed in the oral emtricitabine/tenofovir disoproxil fumarate (F/TDF) active control group (1.69 per 100 person-years, P<0.001) and the oral emtricitabine/tenofovir alafenamide (F/TAF) group (2.02 per 100 person-years).
3. Lenacapavir was well-tolerated, with no new safety signals; while injection-site reactions were common, few participants discontinued the study drug due to these events.
4. The efficacy of daily oral F/TAF was not statistically superior to the calculated background HIV incidence, largely attributed to low adherence among participants.

Study Design

Design
RCT
Double-Blind
Sample
5,345
Patients
Duration
52 weeks
Median
Setting
Multicenter, South Africa and Uganda
Population Adolescent girls and young women aged 16 to 25 years at risk of HIV-1 infection
Intervention Subcutaneous lenacapavir injection every 6 months (with daily oral placebo)
Comparator Daily oral emtricitabine/tenofovir alafenamide (F/TAF) or daily oral emtricitabine/tenofovir disoproxil fumarate (F/TDF) (with subcutaneous placebo)
Outcome Incident HIV-1 infection

Study Limitations

The study used a counterfactual background HIV incidence rate rather than a direct placebo control group, which requires precise modeling and may be sensitive to the assumptions used in its calculation.
The study was limited to cisgender adolescent girls and young women in South Africa and Uganda, potentially limiting the generalizability to other demographics or geographic regions.
Low adherence to daily oral regimens (F/TAF and F/TDF) complicated the assessment of the oral comparators' true biological efficacy.
The interim analysis may not fully capture long-term safety data or rare adverse events associated with extended use of the twice-yearly injectable.

Clinical Significance

This trial represents a potential paradigm shift in HIV prevention, providing a highly effective, long-acting, twice-yearly injectable option that could overcome the adherence challenges associated with daily oral PrEP, particularly for populations at high risk who face significant structural and social barriers.

Historical Context

For over a decade, daily oral F/TDF has been the standard of care for HIV pre-exposure prophylaxis. While effective when taken consistently, real-world adherence has been suboptimal for many high-risk groups. PURPOSE 1 marks the first successful pivotal trial of a six-month, long-acting subcutaneous agent, building on previous advancements in antiretroviral therapy and long-acting HIV injectable treatments.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

What is the unique mechanism of action of lenacapavir compared to traditional antiretrovirals like tenofovir, and why does its pharmacokinetic profile allow for twice-yearly dosing?

Key Response

Lenacapavir is a first-in-class capsid inhibitor that disrupts the HIV-1 viral lifecycle at multiple stages, including capsid-mediated nuclear uptake, virus assembly, and capsid shell formation. Its high potency and extremely low solubility when administered subcutaneously result in a slow-release depot effect, providing sustained therapeutic concentrations for 26 weeks.

Resident
Resident

In the PURPOSE 1 trial, daily oral F/TAF did not show statistical superiority over the background HIV incidence, while lenacapavir showed 100% efficacy. How should this influence your clinical counseling regarding the 'adherence-effectiveness' gap in adolescent girls and young women?

Key Response

The trial highlights that while F/TAF is pharmacologically effective, its real-world efficacy is severely limited by adherence challenges in this demographic. Lenacapavir removes the daily burden of adherence, essentially bridging the gap between 'efficacy' (how it works in a lab) and 'effectiveness' (how it works in a person's life), making it a preferred choice for patients where daily pill-taking is a barrier.

Fellow
Fellow

The study utilized a recency-assay-derived 'counterfactual' background HIV incidence as the primary comparator. What are the implications of this trial design for evaluating the 'pharmacokinetic tail' and the potential for resistance emergence compared to long-acting cabotegravir?

Key Response

Because placebo-controlled trials are now unethical in many regions due to the existence of PrEP, counterfactual modeling is used. However, a major concern with long-acting agents is the 'tail'—the period where drug levels decline below the inhibitory threshold but remain high enough to select for resistance. While lenacapavir has a high genetic barrier, the 100% efficacy in this study means we have not yet seen how the virus escapes lenacapavir pressure in a PrEP-failure context, unlike the INSTI mutations seen with cabotegravir.

Attending
Attending

Given the 100% efficacy result, what are the broader systemic implications for HIV elimination goals, and how do we address the 'zero infections' paradox where a drug is so effective it becomes difficult to study its long-term resistance profile in the field?

Key Response

Lenacapavir represents a potential 'functional vaccine' for high-risk populations. The challenge for clinicians is that with zero breakthrough infections in the trial, we lack data on the specific mutational pathways of resistance in a PrEP setting. We must balance the excitement of high efficacy with the need for rigorous post-marketing surveillance to detect rare breakthroughs and understand the clinical management of the 'long tail' if patients miss doses.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

Critique the validity of using a cross-sectional HIV incidence estimate from the screening cohort (the counterfactual group) as a proxy for the randomized population. What statistical assumptions must hold for this comparison to be unbiased?

Key Response

The validity relies on the 'stationarity' of the epidemic and the assumption that the screening cohort is representative of the enrolled cohort's risk. Potential biases include the 'healthy volunteer effect' (where those who enroll have different behaviors than those who only screen) and the accuracy of the Mean Sleeping Time (MDRI) and False Recent Rate (FRR) parameters used in the recency assay formula to calculate incidence.

Journal Editor
Journal Editor

The PURPOSE 1 trial was stopped early by the Independent Data Monitoring Committee (IDMC). As an editor, what concerns would you have regarding the reporting of secondary endpoints, long-term safety, and the potential overestimation of effect size when a trial is terminated at an interim analysis?

Key Response

Early termination for benefit often leads to an inflation of the treatment effect and may leave the study underpowered for secondary safety endpoints or rare adverse events. Furthermore, stopping early limits our understanding of the durability of the twice-yearly regimen over several years, which is critical for a lifelong prevention strategy.

Guideline Committee
Guideline Committee

Current WHO and CDC guidelines recommend daily oral TDF/FTC or TAF/FTC and bimonthly CAB-LA for PrEP. Based on the PURPOSE 1 data, should lenacapavir be prioritized as a preferred first-line recommendation for cisgender women, and what implementation benchmarks are required before such a change?

Key Response

The 100% efficacy is superior to the historical performance of daily orals in this specific demographic. However, guidelines must consider the 'Implementation Gap'—including cost-effectiveness, the requirement for a healthcare provider to administer the injection every 6 months, and the need for validated HIV testing protocols to avoid starting lenacapavir during an undiagnosed acute infection, which could lead to multi-drug resistance.

Clinical Landscape

Noteworthy Related Trials

2010

iPrEx Trial

n = 2,499 · NEJM

Tested

Daily oral TDF/FTC

Population

MSM and transgender women at high risk for HIV

Comparator

Placebo

Endpoint

Incidence of HIV infection

Key result: Daily oral TDF/FTC reduced the risk of HIV acquisition by 44% compared to placebo in the overall cohort.
2015

PROUD Trial

n = 544 · Lancet

Tested

Daily oral TDF/FTC

Population

MSM at high risk of HIV infection in the UK

Comparator

Deferred PrEP (delayed 12 months)

Endpoint

Confirmed HIV infection

Key result: Daily oral TDF/FTC significantly reduced the risk of HIV acquisition by 86% compared to deferred initiation.
2021

HPTN 084 Trial

n = 3,223 · NEJM

Tested

Injectable Cabotegravir every 8 weeks

Population

Adolescent girls and young women in sub-Saharan Africa

Comparator

Daily oral TDF/FTC

Endpoint

Incident HIV infection

Key result: Injectable cabotegravir was superior to daily oral TDF/FTC, showing an 89% reduction in HIV incidence.

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