New England Journal of Medicine April 20, 2023

Efficacy and Safety of a Bivalent RSV Prefusion F Vaccine in Older Adults

Edward E. Walsh, Gonzalo Pérez Marc, Agnieszka M. Zareba, Ann R. Falsey, Qin Jiang, Michael Patton, Fernando P. Polack, et al.

Bottom Line

In the phase 3 RENOIR trial, a single dose of the bivalent RSVpreF vaccine safely and effectively prevented RSV-associated lower respiratory tract illness in adults 60 years of age and older.

Key Findings

1. Among 34,284 randomized participants, vaccine efficacy against RSV-associated lower respiratory tract illness (LRTI) with at least two signs or symptoms was 66.7% (96.66% CI, 28.8 to 85.8), occurring in 11 participants in the vaccine group (1.19 cases/1000 person-years) compared to 33 in the placebo group (3.58 cases/1000 person-years) [2.1.1].
2. Vaccine efficacy against RSV-associated LRTI with at least three signs or symptoms was 85.7% (96.66% CI, 32.0 to 98.7), representing 2 cases (0.22/1000 person-years) in the vaccine group versus 14 cases (1.52/1000 person-years) in the placebo group.
3. The RSVpreF vaccine also prevented RSV-associated acute respiratory illness with an efficacy of 62.1% (95% CI, 37.1 to 77.9), based on 22 cases in the vaccine group versus 58 in the placebo arm.
4. Safety profiles showed higher incidences of local reactions in the vaccine group compared to placebo (12% vs. 7%), though systemic events were similar between both groups (27% vs. 26%).

Study Design

Design
Phase 3 RCT
Double-Blind
Sample
34,284
Patients
Duration
1 RSV season
Median
Setting
Multicenter, 7 countries
Population Immunocompetent adults 60 years of age or older.
Intervention Single intramuscular injection of a bivalent RSV prefusion F protein-based vaccine (RSVpreF) at a dose of 120 μg (60 μg each for RSV subgroups A and B).
Comparator Single intramuscular injection of matching placebo.
Outcome Vaccine efficacy against seasonal RSV-associated lower respiratory tract illness (LRTI) with at least two, or at least three, signs or symptoms.

Study Limitations

The initial primary analysis only reflected efficacy over a single RSV season, requiring longer-term follow-up to ascertain the durability of protection [1.1.2].
Immunocompromised individuals and those with severe concomitant medical conditions were excluded, limiting generalizability to the highest-risk demographic.
The trial was statistically underpowered to firmly evaluate vaccine efficacy against rare severe outcomes such as RSV-related hospitalization or mortality, due to a low absolute event rate in both study arms.

Clinical Significance

The RENOIR trial was a landmark study that proved the clinical utility of stabilizing the highly immunogenic prefusion F conformation of RSV. By demonstrating high efficacy against symptomatic lower respiratory tract disease in older adults, the trial directly supported the FDA approval of Abrysvo (RSVpreF). Because RSV causes significant seasonal morbidity, hospitalizations, and mortality in populations aged 60 and older, having a safe, efficacious vaccine addresses a major decades-old unmet public health need.

Historical Context

For over 60 years, developing a safe and effective vaccine against RSV remained elusive. The failure of a formalin-inactivated RSV vaccine in the 1960s, which paradoxically caused enhanced respiratory disease and fatalities in children, severely chilled subsequent vaccine research. It was not until the 2010s, when NIH structural biologists successfully mapped and stabilized the metastable prefusion conformation of the RSV F protein—the primary target for highly neutralizing antibodies—that development accelerated. The simultaneous publication of RENOIR (Pfizer's RSVpreF) and AReSVi-006 (GSK's RSVPreF3) in 2023 marked the historic culmination of this effort, breaking the long-standing dry spell in RSV prophylaxis.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

How does targeting the prefusion F protein of RSV confer a more robust and neutralizing immune response compared to targeting the postfusion conformation?

Key Response

The F (fusion) protein is responsible for viral entry into host cells. The prefusion conformation displays highly conserved, highly neutralization-sensitive epitopes (such as Site 0) that are structurally lost once the protein triggers and refolds into the postfusion state. Antibodies generated against the stabilized prefusion F protein are significantly more potent at neutralizing the virus, making this structural biology breakthrough the cornerstone of modern RSV vaccines.

Resident
Resident

When discussing the bivalent RSVpreF vaccine with a 65-year-old patient who has COPD and heart failure, how do you navigate shared clinical decision-making regarding co-administration with seasonal influenza and COVID-19 vaccines?

Key Response

Patients with underlying cardiopulmonary comorbidities are at the highest risk for severe RSV lower respiratory tract illness (LRTI) and stand to benefit the most. Co-administration is clinically safe and practically recommended to maximize adherence before the winter respiratory season, but residents must counsel patients that co-administration can occasionally result in slightly lower immunogenicity or increased local reactogenicity, balancing these minor risks against the danger of delayed or missed vaccination.

Fellow
Fellow

The RSVpreF vaccine is bivalent, covering both RSV A and RSV B subgroups. Given the historical difficulty in achieving balanced immunogenicity, how does the inclusion of both subgroups impact the prevention of RSV-associated LRTI, and what does the RENOIR trial data reveal regarding breakthrough infections by subtype?

Key Response

RSV A and B co-circulate and can alternate dominance seasonally. A bivalent approach ensures broad protection against both strains. Fellows must critically evaluate the trial's subgroup analyses to determine if vaccine efficacy significantly differed between subtypes and consider how potential subtype mismatch could influence seasonal outbreaks in highly vulnerable populations, such as lung transplant recipients or patients with advanced structural lung disease.

Attending
Attending

The FDA approved the RSVpreF vaccine but noted a potential rare risk of Guillain-Barre syndrome (GBS) and atrial fibrillation. How should attendings contextualize these rare safety signals when framing the number needed to vaccinate (NNV) to prevent severe RSV hospitalization in clinical practice?

Key Response

Attendings must weigh the robust efficacy in preventing severe lower respiratory tract illness against rare but serious adverse events. Teaching points should emphasize risk-stratification: the NNV to prevent hospitalization is highly favorable in patients with severe cardiopulmonary disease (where RSV triggers fatal exacerbations), justifying a strong recommendation, whereas a more nuanced shared decision-making approach is appropriate for healthy 60-year-olds with lower baseline risk.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

The RENOIR trial utilized a case-driven, event-triggered design rather than a fixed follow-up period to determine its primary efficacy endpoints. What are the statistical advantages and potential biases of this approach in evaluating a seasonal respiratory virus vaccine?

Key Response

Event-driven designs maximize statistical power by ensuring a sufficient number of primary endpoints (e.g., severe RSV LRTI) are captured, which accelerates trial completion. However, this approach can introduce temporal bias if the follow-up period truncates midway through a respiratory season, potentially misestimating the waning of immunity and the long-term durability of vaccine efficacy across multiple distinct seasons.

Journal Editor
Journal Editor

The primary endpoint in the RENOIR trial relied on specific clinical symptoms combined with PCR-confirmed RSV. How might the sensitivity of these symptom definitions for lower respiratory tract illness (LRTI) impact the calculated vaccine efficacy, and what methodological gaps exist regarding endpoint ascertainment in frail or cognitively impaired older adults?

Key Response

Subjective symptom reporting in older adults, especially those with baseline dyspnea from conditions like COPD or cognitive impairment, can lead to misclassification bias. A critical reviewer would flag that if the case definition requires acute symptoms often masked by chronic illness, the trial might systematically miss cases or artificially inflate vaccine efficacy against narrowly defined LRTI compared to broader, real-world all-cause RSV hospitalizations.

Guideline Committee
Guideline Committee

Based on the RENOIR trial's efficacy data and subsequent real-world cost-effectiveness analyses, should ACIP guidelines formally transition from a 'shared clinical decision-making' recommendation to a universal age-based recommendation (e.g., all adults >75 or >60 with risk factors), and how do these findings compare to existing influenza guidelines?

Key Response

Initially, ACIP recommended RSV vaccines for adults >60 based on shared clinical decision-making due to high cost per QALY and the rare GBS signal. Evaluating RENOIR's robust protection against LRTI alongside the substantial disease burden has led to guideline updates (e.g., universally recommending it for >75 and at-risk >60). The committee must constantly weigh NNV, durability of protection, and healthcare economics to justify moving from individualized to categorical recommendations, similar to established universal age-based influenza vaccine guidelines.

Clinical Landscape

Noteworthy Related Trials

2023

AReSVi-006 Trial

n = 24,966 · NEJM

Tested

RSVPreF3 OA vaccine

Population

Adults 60 years of age or older

Comparator

Placebo

Endpoint

RSV-associated lower respiratory tract disease (LRTD)

Key result: The vaccine showed 82.6% efficacy against RSV-associated LRTD and was generally well tolerated.
2023

ConquerRSV Trial

n = 35,541 · NEJM

Tested

mRNA-1345 RSV vaccine

Population

Adults 60 years of age or older

Comparator

Placebo

Endpoint

RSV-associated LRTD with at least 2 symptoms

Key result: The mRNA vaccine demonstrated 83.7% efficacy against RSV-associated LRTD with at least two signs or symptoms.
2023

MATISSE Trial

n = 7,358 · NEJM

Tested

Bivalent RSVpreF vaccine

Population

Pregnant women at 24 to 36 weeks gestation

Comparator

Placebo

Endpoint

Severe medically attended RSV-associated LRTD in infants

Key result: Maternal vaccination yielded 81.8% efficacy against severe infant RSV-associated LRTD within 90 days of birth.

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