The New England Journal of Medicine MAY 27, 2004

A Comparison of Albumin and Saline for Fluid Resuscitation in the Intensive Care Unit

The SAFE Study Investigators

Bottom Line

The landmark SAFE trial demonstrated that the use of 4 percent albumin is as safe as normal saline for fluid resuscitation in a heterogeneous population of critically ill patients, with no significant difference in 28-day mortality.

Key Findings

1. There was no significant difference in 28-day all-cause mortality between the albumin group (20.9%) and the saline group (21.1%) (Relative Risk, 0.99; 95% CI, 0.91-1.09; P=0.87).
2. Secondary clinical outcomes, including duration of ICU and hospital stay, requirement for mechanical ventilation, and duration of renal replacement therapy, were not significantly different between the two treatment arms.
3. The incidence of new single-organ or multiple-organ failure did not differ significantly between the groups (P=0.85).
4. Post-hoc subgroup analyses suggested potential safety concerns in patients with traumatic brain injury (increased mortality risk) and potential benefits in patients with septic shock, though these findings were not the study's primary objective.

Study Design

Design
RCT
Double-Blind
Sample
6,997
Patients
Duration
28 d
Median
Setting
Multicenter, international
Population Critically ill patients admitted to the ICU who required fluid resuscitation
Intervention Intravenous 4 percent albumin
Comparator 0.9 percent normal saline
Outcome All-cause mortality at 28 days

Study Limitations

The heterogeneous nature of the ICU population may have diluted potential treatment effects that could exist within specific clinical subgroups.
The trial was not powered to detect mortality differences in pre-specified subgroups, such as patients with traumatic brain injury or septic shock.
The results are specific to 4% albumin and cannot be extrapolated to hyper-oncotic albumin solutions or synthetic colloids.
The study did not evaluate long-term outcomes beyond 28 days.

Clinical Significance

The SAFE trial resolved decades of controversy regarding the superiority of colloids over crystalloids for routine ICU resuscitation. It established that albumin is a safe, albeit significantly more expensive, alternative to normal saline, reinforcing that the choice of fluid should be guided by cost and specific patient physiology rather than a presumed survival advantage.

Historical Context

Prior to the SAFE trial, the use of albumin in critically ill patients was highly polarized, fueled by a 1998 meta-analysis suggesting an increased mortality risk associated with albumin use. This randomized trial served as a definitive intervention to address these safety concerns, ultimately leading to a more nuanced, evidence-based approach to fluid selection in critical care.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

Based on the Starling equation and the principles of fluid dynamics, why was it hypothesized that 4% albumin would be superior to 0.9% saline for intravascular volume expansion, and what ratio of saline-to-albumin volume was actually observed in the SAFE trial?

Key Response

Albumin provides colloid oncotic pressure which theoretically keeps fluid within the intravascular compartment, unlike crystalloids which equilibrate with the interstitium. While the traditional teaching suggested a 1:3 or 1:4 ratio, the SAFE trial found a much lower ratio of approximately 1:1.4, indicating that the 'volume-sparing' effect of colloids is less pronounced in critically ill patients with increased capillary permeability than in healthy models.

Resident
Resident

The SAFE trial conducted several pre-defined subgroup analyses. Which specific patient population showed a significant increase in 28-day mortality when resuscitated with albumin, and how should this influence your management of a multi-trauma patient?

Key Response

Patients with traumatic brain injury (TBI) had significantly higher mortality rates when treated with albumin compared to saline (later confirmed in the SAFE-TBI follow-up study). It is hypothesized that albumin may increase intracranial pressure in the setting of a disrupted blood-brain barrier. Therefore, normal saline remains the gold standard for volume resuscitation in neuro-trauma patients.

Fellow
Fellow

Compare the renal safety profile of albumin in the SAFE trial with the findings for synthetic colloids like Hydroxyethyl Starch (HES) in the later CHEST and 6S trials. How does this distinction shape the 'colloid vs. crystalloid' debate in modern intensive care?

Key Response

Unlike synthetic colloids (HES), which were found to increase the risk of acute kidney injury and the need for renal replacement therapy (RRT), the SAFE trial showed no difference in RRT or new organ failure between albumin and saline. This demonstrates that 'colloids' are not a homogenous class; natural colloids like albumin lack the nephrotoxicity associated with starches, maintaining a role in certain clinical phenotypes despite the neutral primary mortality outcome.

Attending
Attending

Given that the SAFE trial found no difference in 28-day mortality, yet albumin is significantly more expensive than saline, in which specific clinical scenarios do you believe the 'volume-sparing' effect of albumin justifies its cost in your practice?

Key Response

This question addresses the move from 'survival' to 'morbidity' endpoints. In patients where fluid overload is particularly detrimental—such as those with severe ARDS, precarious cardiac output, or those already significantly fluid-positive—clinicians may choose albumin to achieve hemodynamic stability with lower total volume, despite the lack of a mortality benefit in the general ICU population.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

The SAFE trial is lauded for its pragmatic design and high internal validity. Evaluate the implications of the 'masked' fluid administration technique used (identical opaque bags) and discuss how the choice of a 28-day mortality primary endpoint might miss late-occurring complications or benefits related to fluid choice.

Key Response

The use of identical bags and tubing was a methodological triumph that prevented clinician bias in fluid titration. However, 28-day mortality is a relatively short-term 'hard' endpoint. Researching fluid resuscitation may require longer-term follow-up (e.g., 90 days or 1 year) or functional recovery metrics to identify subtle differences in organ recovery or systemic inflammation that do not manifest as early death.

Journal Editor
Journal Editor

As a reviewer, how would you address the discrepancy between the SAFE trial's neutral primary outcome and the 'near-significant' (p=0.09) mortality benefit observed in the sepsis subgroup? Should the authors have been allowed to emphasize this in the abstract?

Key Response

This is a classic 'multiplicity' problem in large trials. Editors must ensure that subgroup findings are presented as hypothesis-generating only. Over-emphasizing a p=0.09 subgroup result in the abstract can lead to premature clinical adoption. The SAFE authors correctly maintained a neutral stance, which eventually led to the ALBIOS trial specifically designed to test the sepsis hypothesis.

Guideline Committee
Guideline Committee

How do the findings of the SAFE trial and subsequent meta-analyses influence the Surviving Sepsis Campaign (SSC) recommendations regarding the use of albumin versus crystalloids for initial resuscitation?

Key Response

Current SSC guidelines (2021) provide a 'weak recommendation' with 'moderate-quality evidence' for using albumin in patients who received large volumes of crystalloids. This is heavily influenced by the SAFE trial's sepsis subgroup and the ALBIOS trial. While crystalloids remain the first-line fluid of choice (Strong Recommendation), albumin is the only colloid endorsed for use when crystalloid volumes are high, distinguishing it from HES and gelatins which are recommended against.

Clinical Landscape

Noteworthy Related Trials

2012

CHEST Trial

n = 7,000 · NEJM

Tested

6% Hydroxyethyl starch (HES)

Population

ICU patients requiring fluid resuscitation

Comparator

0.9% Saline

Endpoint

90-day mortality

Key result: The use of hydroxyethyl starch for fluid resuscitation in patients in the ICU was associated with an increased risk of renal-replacement therapy.
2013

CRISTAL Trial

n = 2,857 · NEJM

Tested

Colloids (albumin, gelatin, starches)

Population

ICU patients with hypovolemia

Comparator

Crystalloids (saline, Ringer's lactate)

Endpoint

28-day mortality

Key result: There was no statistically significant difference in 28-day mortality between patients treated with colloids and those treated with crystalloids.
2018

SMART Trial

n = 15,802 · NEJM

Tested

Balanced crystalloids (e.g., Plasma-Lyte, Lactated Ringer's)

Population

Critically ill adults in the ICU

Comparator

0.9% Saline

Endpoint

Major adverse kidney events within 30 days (MAKE30)

Key result: Among critically ill adults, the use of balanced crystalloids reduced the composite outcome of death, new renal-replacement therapy, or persistent renal dysfunction compared to saline.

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