New England Journal of Medicine March 28, 2019

A Trial of a Shorter Regimen for Rifampin-Resistant Tuberculosis

Andrew J. Nunn, Patrick P.J. Phillips, Sarah K. Meredith et al.

Bottom Line

A 9-to-11-month short regimen for rifampin-resistant tuberculosis was noninferior to the conventional 20-month WHO-recommended regimen in achieving a favorable outcome at 132 weeks, with similar rates of severe adverse events but a higher incidence of QT prolongation.

Key Findings

1. Favorable status at 132 weeks was achieved in 78.8% of participants in the short-regimen group versus 79.8% in the long-regimen group.
2. The adjusted difference in favorable status was 1.0 percentage point (95% CI, -7.5 to 9.5), successfully meeting the noninferiority margin of 10 percentage points (P=0.02 for noninferiority).
3. Adverse events of grade 3 or higher occurred at comparable rates: 48.2% in the short-regimen group and 45.4% in the long-regimen group.
4. Prolongation of the QT or corrected QT interval (QTcF) to 500 msec or more was notably more frequent in the short-regimen group (11.0%) compared to the long-regimen group (6.4%).
5. Acquired resistance to fluoroquinolones or aminoglycosides was similar between the short regimen (3.3%) and the long regimen (2.3%).

Study Design

Design
Randomized Controlled Trial
Open-Label
Sample
424
Patients
Duration
132 wk
Median
Setting
Multicenter, International
Population Patients with rifampin-resistant tuberculosis that was susceptible to fluoroquinolones and aminoglycosides.
Intervention Short regimen (9 to 11 months) including high-dose moxifloxacin, clofazimine, ethambutol, pyrazinamide, kanamycin, isoniazid, and prothionamide.
Comparator Long regimen (20 months) following the 2011 WHO guidelines for MDR-TB.
Outcome Favorable status at 132 weeks, defined by cultures negative for Mycobacterium tuberculosis at 132 weeks and at a previous occasion, with no intervening positive culture or previous unfavorable outcome.

Study Limitations

The open-label design of the trial could introduce bias in clinical management decisions and the reporting of subjective adverse events.
The short regimen still relied on a second-line injectable agent (kanamycin), which carries significant risks of toxicity, such as irreversible ototoxicity.
Higher rates of QT prolongation in the short-regimen arm necessitate intensive electrocardiographic monitoring, which may be challenging to implement in resource-limited settings.
The study excluded patients with baseline resistance to fluoroquinolones or second-line aminoglycosides, limiting the generalizability to extensively drug-resistant tuberculosis (XDR-TB) populations.

Clinical Significance

The STREAM Stage 1 trial provided the first rigorous randomized controlled evidence that a substantially shortened (9-11 month) regimen for multidrug-resistant tuberculosis (MDR-TB) is noninferior to the traditionally lengthy and poorly tolerated 20-month standard of care. By validating the efficacy of the modified 'Bangladesh regimen,' this trial catalyzed a paradigm shift in global MDR-TB guidelines, demonstrating that treatment could be shortened to reduce patient burden and healthcare costs. It also highlighted the critical need for cardiac monitoring due to the cumulative QT-prolonging effects of high-dose moxifloxacin and clofazimine, paving the way for the development of the next generation of safer, all-oral shortened regimens.

Historical Context

Historically, MDR-TB treatment relied on 20-to-24-month regimens comprising multiple toxic second-line drugs, including prolonged use of injectables. These regimens were associated with high costs, severe adverse effects, poor adherence, and global treatment success rates of only around 50%. In the late 2000s, observational cohort studies in Bangladesh pioneered a highly successful 9-month regimen (the 'Bangladesh regimen'). The STREAM Stage 1 trial was launched to formally evaluate this approach in a phase 3 multicenter noninferiority trial. Prompted by preliminary STREAM data, the WHO conditionally recommended a shorter 9-to-12-month regimen in 2016. The final 2019 STREAM publication confirmed the validity of this approach and set the foundation for subsequent trials (like STREAM Stage 2, TB-PRACTECAL, and Nix-TB/ZeNix) that replaced injectable agents with novel oral drugs like bedaquiline and pretomanid.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

Why is QT prolongation a specific concern in the shorter regimens for rifampin-resistant TB, and what classes of medications included in these regimens are most responsible for this adverse effect?

Key Response

Tests foundational pharmacology. Fluoroquinolones (like high-dose moxifloxacin) and clofazimine are cornerstone drugs for MDR-TB that inhibit bacterial DNA gyrase and mycobacterial ATP synthase, respectively, but share the critical adverse effect of prolonging the QTc interval, requiring baseline and ongoing ECG monitoring.

Resident
Resident

A patient initiated on the 9-to-11-month STREAM regimen develops a QTc interval of 520 ms on routine ECG monitoring at week 4. They are asymptomatic. How should this be managed clinically while balancing the risk of MDR-TB treatment failure?

Key Response

Residents need to manage toxicities. A QTc over 500 ms usually requires holding the offending agents (e.g., moxifloxacin, clofazimine), checking and correcting electrolytes, and re-evaluating the regimen once the QTc normalizes. This is a critical clinical decision to avoid Torsades de Pointes while maintaining anti-mycobacterial efficacy.

Fellow
Fellow

The STREAM trial established the non-inferiority of the shorter regimen, but subsequent WHO guidance has shifted towards all-oral regimens over the injectable-containing short regimen used in this trial. How do we integrate the STREAM findings into the current landscape of bedaquiline-sparing versus bedaquiline-containing all-oral short regimens?

Key Response

Fellows must know the evolution of regimens. The original STREAM trial used an injectable (kanamycin). Newer iterations (STREAM stage 2) and current practice emphasize all-oral bedaquiline regimens. Understanding how the non-inferiority of a short duration paved the way for short all-oral regimens is key fellowship-level knowledge.

Attending
Attending

Given that the shorter regimen had a higher rate of QT prolongation but comparable overall severe adverse events and a much lower burden of treatment, how do you counsel a newly diagnosed MDR-TB patient regarding the trade-offs of these regimens to maximize adherence?

Key Response

Attendings focus on shared decision-making. A 20-month regimen has immense psychosocial and economic toxicity, often leading to loss to follow-up. The shorter regimen trades duration for intensive cardiac monitoring. Teaching residents to weigh programmatic success and patient-centered care against specific drug toxicities is paramount.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

In assessing the non-inferiority of the 9-11 month regimen at 132 weeks, the choice of the non-inferiority margin and the handling of missing data are critical. How does the choice of a modified intention-to-treat versus a per-protocol analysis impact the validity of a non-inferiority claim in a trial with high potential for treatment discontinuation?

Key Response

In non-inferiority trials, ITT analysis can bias results toward the null (making treatments look similar), so both ITT and per-protocol analyses are usually required. High dropout rates in 20-month TB regimens complicate this, making the statistical handling of missing data a pivotal methodological critique.

Journal Editor
Journal Editor

As an editor evaluating the STREAM trial manuscript, what are the primary threats to external validity regarding the control arm (the 20-month WHO regimen), and how might the pragmatic, unblinded nature of the trial have influenced the reporting of subjective adverse events or loss to follow-up?

Key Response

Unblinded trials with vastly different treatment durations are highly susceptible to performance bias and differential loss to follow-up. Furthermore, the standard 20-month regimen often performs poorly in real-world settings due to adherence issues, so an editor would scrutinize whether the control arm performed as expected or artificially inflated comparative success.

Guideline Committee
Guideline Committee

Based on the STREAM trial evidence demonstrating non-inferiority but higher rates of QT prolongation, should the WHO guidelines universally recommend this shorter regimen over the 20-month regimen, and what health-system prerequisites must be mandated in the recommendation?

Key Response

Guideline development requires pairing recommendations with resource requirements. The WHO updated its guidelines to recommend shorter regimens for MDR-TB, but explicitly noted the need for active pharmacovigilance and ECG monitoring due to the fluoroquinolone and clofazimine components. The committee must weigh efficacy against the conditional need for monitoring infrastructure.

Clinical Landscape

Noteworthy Related Trials

2020

Nix-TB Trial

n = 109 · NEJM

Tested

Bedaquiline, pretomanid, and linezolid (BPaL) for 6 months

Population

Patients with extensively drug-resistant (XDR) or treatment-intolerant/nonresponsive MDR-TB

Comparator

None (Single-arm study)

Endpoint

Favorable outcome (resolution of clinical disease and negative culture) at 6 months post-treatment

Key result: 90 percent of patients had a favorable outcome at 6 months after the end of treatment, demonstrating high efficacy of a 6-month all-oral regimen.
2022

TB-PRACTECAL Trial

n = 552 · NEJM

Tested

24-week all-oral regimens containing bedaquiline, pretomanid, linezolid, and moxifloxacin (BPaLM)

Population

Patients with rifampin-resistant tuberculosis

Comparator

Locally accepted standard of care (9 to 24 month regimens)

Endpoint

Percentage of patients with an unfavorable outcome (death, treatment failure, discontinuation, or loss to follow-up) at 72 weeks

Key result: The BPaLM regimen was noninferior and superior to the standard of care, with an unfavorable outcome occurring in 11 percent versus 48 percent of patients.
2022

ZeNix Trial

n = 181 · NEJM

Tested

BPaL regimen with reduced linezolid doses (1200 mg or 600 mg) and durations (26 or 9 weeks)

Population

Patients with highly drug-resistant tuberculosis

Comparator

Internal comparisons of different linezolid dosages and durations within the BPaL regimen

Endpoint

Favorable clinical outcome at 6 months after the end of treatment

Key result: High rates of favorable outcomes (84 to 93 percent) were achieved across all groups, with lower doses and shorter durations of linezolid significantly reducing toxic effects like peripheral neuropathy.

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