The Lancet September 07, 2026

Use of the bacterial lysate OM-85 for the primary prevention of wheezing lower respiratory illness in preschool children: a randomised, placebo-controlled trial

Wayne J Morgan, David T Mauger, Leonard B Bacharier, et al.

Bottom Line

In a randomized trial of high-risk preschool children, prophylactic oral administration of the bacterial lysate OM-85 for 24 months did not significantly reduce the time to first wheezing lower respiratory tract illness during a subsequent 36-month off-drug observation period.

Key Findings

1. Among the 822 randomized infants, 132 experienced at least one wheezing lower respiratory tract illness during the 36-month off-drug observation period.
2. The incidence of at least one wheezing illness during observation was 21% (71 of 342) in the OM-85 group and 18% (61 of 339) in the placebo group.
3. There was no significant difference between OM-85 and placebo regarding the time to first wheezing lower respiratory tract illness (hazard ratio 1.16 [95% CI 0.82-1.64]; log-rank p=0.35).
4. The most frequent adverse events, including fevers, coughs, and colds, did not differ between the intervention and placebo groups.

Study Design

Design
RCT
Double-Blind
Sample
822
Patients
Duration
60 mo
Median
Setting
Multicenter, US
Population Children aged 6-18 months at increased risk for asthma due to atopic dermatitis, parental asthma, or asthma in a blood sibling aged 4 years or older
Intervention Oral OM-85 3.5 mg for 10 days each month for 24 months
Comparator Matching placebo for 10 days each month for 24 months
Outcome Time to first wheezing lower respiratory tract illness during the 36-month observation period off the study drug

Study Limitations

A notable attrition rate over the prolonged trial duration, with 822 children randomized but only 596 completing the full 36-month observation period.
The primary outcome was assessed during an off-drug period to test for disease modification/primary prevention, meaning it does not evaluate potential short-term efficacy for acute symptom control while actively receiving the drug.
The cohort was exclusively selected for being at high risk for asthma (due to atopic dermatitis or family history), so the findings may not fully generalize to average-risk populations.

Clinical Significance

This trial robustly demonstrates that early-life administration of the bacterial lysate OM-85 does not alter the underlying disease trajectory or prevent asthma-like symptoms in high-risk infants, discouraging its clinical use for the primary prevention of wheezing lower respiratory tract illnesses.

Historical Context

Recurrent wheezing in preschool children is a major driver of pediatric healthcare utilization and a precursor to asthma. Inspired by the 'hygiene hypothesis,' researchers have extensively investigated whether early-life immunomodulation via microbial exposure can prevent allergic diseases. While earlier, smaller studies suggested bacterial extracts like OM-85 might reduce the frequency of acute respiratory infections, the ORBEX trial rigorously tested the long-term, disease-modifying potential of OM-85 for the primary prevention of wheezing. Its negative results cast significant doubt on the viability of this pathway for asthma prevention.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

What is the proposed immunologic mechanism by which a bacterial lysate like OM-85 was originally theorized to prevent recurrent wheezing or asthma development in preschool children?

Key Response

This tests foundational immunology and the hygiene hypothesis. Bacterial lysates were theorized to stimulate innate immune receptors (like Toll-like receptors) on mucosal surfaces, promoting a shift from a Th2 (allergic/asthma) phenotype toward a Th1 and regulatory T-cell response, thereby theoretically reducing atopic sensitization and subsequent viral-induced wheezing.

Resident
Resident

Given the negative findings of this trial, how should you counsel parents of a high-risk preschooler with recurrent wheezing who ask about using prescription or over-the-counter immunostimulants to prevent asthma?

Key Response

Residents must translate trial data into practical patient counseling. The rationale is to definitively advise against the use of OM-85 for primary prevention due to a lack of long-term efficacy, and instead pivot the conversation to guideline-directed therapies such as PRN or daily inhaled corticosteroids (ICS) or leukotriene receptor antagonists for those with frequent symptoms.

Fellow
Fellow

The trial specifically evaluated a 36-month off-drug observation period after 24 months of therapy to assess primary prevention. Why is distinguishing between primary disease modification and secondary exacerbation reduction critical when evaluating therapies in the heterogeneous population of preschool wheezers?

Key Response

Fellows must understand disease phenotyping and trial endpoints. Preschool wheeze is highly heterogeneous (transient viral wheeze vs. multiple-trigger atopic asthma). An intervention might temporarily reduce viral infections (secondary prevention) while actively on-drug, but failing to delay the time to first wheeze off-drug indicates it does not fundamentally alter the underlying immune trajectory or natural history of the child's disease.

Attending
Attending

OM-85 has been prescribed in various global regions for decades based on early, less rigorous data and biological plausibility. How does this large, negative, long-term RCT impact our approach to de-adopting legacy therapies in pediatric pulmonology?

Key Response

This highlights the challenge of de-implementation in clinical practice. The rationale focuses on teaching trainees to demand high-quality, long-term RCTs for disease-modifying claims, and recognizing the importance of stopping ineffective, low-value care even if the intervention is perceived as safe or mechanistically sound.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

In a 5-year longitudinal trial (2 years on-drug, 3 years off-drug) assessing time to first wheezing lower respiratory illness, how might interval censoring and informative dropout threaten the validity of the Kaplan-Meier estimates and Cox proportional hazards models used?

Key Response

This focuses on the statistical methodology of long-term pediatric trials. Dropout rates over 5 years are often high. If children drop out because they develop severe symptoms and seek outside treatments, or conversely because they are entirely healthy and parents lose interest, the censoring is informative. This violates the fundamental assumption of survival analysis and potentially biases the hazard ratio.

Journal Editor
Journal Editor

As a peer reviewer evaluating this manuscript, what critical data regarding medication adherence during the 24-month prophylactic phase and the specific phenotypic definition of the high-risk cohort would you scrutinize to ensure the null result is a true lack of efficacy rather than a type II error?

Key Response

Reviewers must look for methodological flaws that cause false negatives. Poor adherence over a 2-year daily regimen naturally biases results toward the null. Furthermore, if the high-risk inclusion criteria were too broad, including many children destined to outgrow transient wheeze regardless of intervention, it would severely dilute any potential preventative signal.

Guideline Committee
Guideline Committee

Based on this definitive lack of long-term efficacy, how should organizations like GINA (Global Initiative for Asthma) update their recommendations regarding the use of bacterial lysates for the primary prevention of preschool wheezing?

Key Response

Current GINA guidelines note that while some earlier studies suggested bacterial lysates might reduce viral respiratory infections, their routine use is not recommended for asthma management. This study provides robust, high-level evidence to strengthen that stance to a strong, formal recommendation against the use of OM-85 for the primary prevention of wheezing illnesses in high-risk preschoolers, directing clinical focus back to proven therapies.

Clinical Landscape

Noteworthy Related Trials

2006

PEAK Trial

n = 285 · NEJM

Tested

Inhaled fluticasone propionate

Population

Preschoolers at high risk for asthma

Comparator

Placebo

Endpoint

Proportion of episode-free days

Key result: Inhaled corticosteroids did not alter the natural history of asthma or prevent future wheezing episodes after discontinuation.
2010

Razi OM-85 Trial

n = 75 · JACI

Tested

OM-85 bacterial lysate

Population

Children aged 1 to 6 years with recurrent wheezing

Comparator

Placebo

Endpoint

Number and duration of wheezing attacks

Key result: OM-85 significantly reduced both the number and duration of wheezing attacks compared to placebo.
2015

APRIL Trial

n = 607 · JAMA

Tested

Azithromycin

Population

Children 12 to 71 months with recurrent severe LRTI

Comparator

Placebo

Endpoint

Progression to severe lower respiratory tract illness

Key result: Early administration of azithromycin significantly reduced the risk of progressing to severe LRTI during viral infections.

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