New England Journal of Medicine July 17, 2025

Perioperative Durvalumab in Gastric and Gastroesophageal Junction Cancer

Yelena Y Janjigian, Salah-Eddin Al-Batran, Zev A Wainberg, et al.

Bottom Line

In patients with resectable gastric or gastroesophageal junction adenocarcinoma, the addition of perioperative durvalumab to standard FLOT chemotherapy significantly improved event-free survival compared with chemotherapy alone.

Key Findings

1. At a median follow-up of 31.5 months, perioperative durvalumab significantly reduced the risk of disease progression, recurrence, or death by 29% compared to chemotherapy alone (HR 0.71; 95% CI 0.58-0.86; P<0.001).
2. The 2-year event-free survival rate was 67.4% in the durvalumab plus FLOT group versus 58.5% in the placebo plus FLOT group.
3. Median event-free survival was not yet reached in the durvalumab arm, compared to 32.8 months in the placebo arm.
4. The safety profile of durvalumab plus FLOT was consistent with the known profiles of the individual agents, and the addition of durvalumab did not compromise surgical feasibility or increase major perioperative morbidity.

Study Design

Design
Phase 3 RCT
Double-Blind
Sample
948
Patients
Duration
31.5 mo
Median
Setting
20 countries
Population Patients aged 18 years or older with previously untreated, histologically documented, resectable (stage II-IVA) gastric or gastroesophageal junction (GEJ) adenocarcinoma.
Intervention Durvalumab (1500 mg every 4 weeks) plus FLOT (fluorouracil, leucovorin, oxaliplatin, docetaxel) for 4 perioperative cycles (2 neoadjuvant, 2 adjuvant), followed by adjuvant durvalumab for 10 cycles.
Comparator Placebo every 4 weeks plus FLOT for 4 perioperative cycles, followed by adjuvant placebo for 10 cycles.
Outcome Event-free survival (EFS) assessed by blinded independent central review and/or local pathology testing.

Study Limitations

Overall survival (OS) data, a key secondary endpoint, were not yet mature at the time of this primary event-free survival analysis.
Black patients were substantially underrepresented in the study cohort (accounting for approximately 1%), potentially limiting the generalizability of the findings across diverse populations.
The study design does not allow for distinguishing the relative efficacy contribution of the neoadjuvant chemoimmunotherapy phase versus the extended adjuvant durvalumab monotherapy phase.

Clinical Significance

The MATTERHORN trial establishes a new standard of care for patients with early-stage, resectable gastric and gastroesophageal junction adenocarcinoma. By demonstrating that the addition of the PD-L1 inhibitor durvalumab to perioperative FLOT chemotherapy yields a statistically significant and clinically meaningful improvement in event-free survival, the study validates the integration of immunotherapy into the curative-intent setting for these high-risk malignancies.

Historical Context

Historically, the landmark FLOT4 trial established perioperative FLOT (fluorouracil, leucovorin, oxaliplatin, and docetaxel) as the standard of care for resectable gastric and gastroesophageal junction cancers, offering improved survival over older anthracycline-based regimens. However, despite optimal surgery and chemotherapy, recurrence rates remained frustratingly high. Concurrently, immune checkpoint inhibitors revolutionized the management of advanced/metastatic gastrointestinal cancers, as demonstrated in trials like CheckMate-649 (nivolumab) and KEYNOTE-859 (pembrolizumab). The phase 3 MATTERHORN trial was designed to bridge this gap, testing whether introducing PD-L1 blockade earlier into the perioperative, curative-intent setting could effectively reduce recurrences and improve long-term outcomes.

Guided Discussion

High-yield insights from every perspective

Med Student
Medical Student

What is the mechanism of action of durvalumab, and why is it theoretically synergistic with the FLOT chemotherapy regimen in the treatment of resectable gastric adenocarcinoma?

Key Response

Durvalumab is a monoclonal antibody that blocks PD-L1, preventing it from binding to PD-1 and CD80, thereby releasing T-cell suppression. FLOT chemotherapy induces immunogenic cell death, releasing tumor neoantigens and upregulating inflammatory pathways, which primes the tumor microenvironment for enhanced T-cell activation when immune checkpoint inhibition is applied.

Resident
Resident

When managing a patient receiving perioperative durvalumab and FLOT, what are the components of the FLOT regimen, and how does the addition of immunotherapy alter the expected perioperative toxicity profile and surgical timing?

Key Response

FLOT comprises 5-fluorouracil, leucovorin, oxaliplatin, and docetaxel. While the MATTERHORN trial showed similar rates of surgical complications, the addition of durvalumab introduces the risk of immune-related adverse events (irAEs) like pneumonitis, colitis, or endocrinopathies, which require high vigilance as they can complicate surgical recovery or delay the planned resection.

Fellow
Fellow

The MATTERHORN trial demonstrated an event-free survival benefit with the addition of durvalumab. How does a patient's mismatch repair (MMR) status and PD-L1 Combined Positive Score (CPS) influence the expected magnitude of benefit from this regimen, and how do these findings contrast with the recent KEYNOTE-585 trial?

Key Response

Patients with mismatch repair deficient (dMMR) or microsatellite instability-high (MSI-H) tumors typically derive the most profound benefit from immune checkpoint inhibitors. Evaluating if PD-L1 CPS cutoffs dictate efficacy remains debated, especially since KEYNOTE-585 (pembrolizumab plus FLOT) failed to meet its primary EFS endpoint, raising questions about trial design differences, drug-specific effects, and optimal patient selection.

Attending
Attending

Although the MATTERHORN trial met its primary endpoint of event-free survival, overall survival data remains immature. Given this, how should clinicians counsel a fit patient with newly diagnosed resectable gastric cancer about incorporating durvalumab into their FLOT regimen, particularly regarding the risk of long-term immune toxicities versus uncertain survival benefit?

Key Response

In gastrointestinal oncology, EFS and pathologic complete response (pCR) are strong surrogates but do not always translate to an overall survival (OS) benefit. Attendings must balance the proven early surrogate benefits against the permanent risks of immunotherapy (e.g., lifelong adrenal insufficiency or hypothyroidism) and discuss these trade-offs transparently until mature OS data is available.

Scholarly Review

Critical appraisal through the lens of expert reviewers and guideline development

PhD
PhD

In the MATTERHORN trial design, event-free survival (EFS) was chosen as the primary endpoint. From a methodological standpoint, how does the inclusion of 'progression precluding surgery' in the EFS definition impact statistical power and the interpretation of the results compared to traditional disease-free survival (DFS)?

Key Response

EFS captures events that occur before surgery, such as disease progression during neoadjuvant therapy or failure to reach resection, which DFS misses (as DFS only applies to patients who undergo surgery). Capturing these early events provides a more comprehensive measure of neoadjuvant efficacy and increases early event rates, boosting statistical power, though it complicates direct comparisons with older adjuvant-focused trials.

Journal Editor
Journal Editor

As a peer reviewer evaluating the MATTERHORN manuscript, what critical scrutiny must be applied to the geographical distribution of the enrolled patients, specifically regarding regional variations in surgical techniques like D2 lymphadenectomy and baseline tumor biology?

Key Response

Gastric cancer biology and surgical standards vary significantly by region; for instance, Asian centers traditionally perform more extensive D2 lymphadenectomies and see higher rates of distal tumors compared to Western centers. A seasoned reviewer would flag whether the survival benefit of durvalumab is consistent across these regions, as imbalances in surgical quality could confound generalizability.

Guideline Committee
Guideline Committee

NCCN and ESMO guidelines currently recommend perioperative FLOT as a preferred standard for resectable gastric and GEJ adenocarcinoma. Based on the MATTERHORN data, should the guidelines be updated to universally recommend the addition of durvalumab, or should its use be restricted based on specific biomarkers like MSI status or PD-L1 CPS?

Key Response

While the addition of durvalumab improves EFS, updating guidelines to a universal strong recommendation typically requires mature Overall Survival (OS) data. Committee members must debate whether to issue a category 2A recommendation for the overall population based on EFS and pCR improvements, while possibly making a stronger recommendation for the dMMR/MSI-H subgroup where immunotherapy has a well-established, outsized benefit.

Clinical Landscape

Noteworthy Related Trials

2019

FLOT4-AIO Trial

n = 716 · Lancet

Tested

Perioperative FLOT chemotherapy

Population

Patients with resectable gastric or GEJ cancer

Comparator

Perioperative ECF or ECX chemotherapy

Endpoint

Overall survival

Key result: Perioperative FLOT significantly prolonged overall survival compared to ECF/ECX regimens.
2021

CheckMate 577

n = 794 · NEJM

Tested

Adjuvant nivolumab

Population

Patients with resected esophageal or GEJ cancer after neoadjuvant chemoradiotherapy

Comparator

Placebo

Endpoint

Disease-free survival

Key result: Adjuvant nivolumab doubled median disease-free survival compared to placebo in patients with residual pathologic disease.
2024

KEYNOTE-585 Trial

n = 1007 · Lancet Oncol

Tested

Perioperative pembrolizumab plus chemotherapy

Population

Patients with locally advanced resectable gastric or GEJ cancer

Comparator

Placebo plus chemotherapy

Endpoint

Event-free survival and pathological complete response

Key result: Pembrolizumab significantly improved pathological complete response rates but did not meet the statistical threshold for improved event-free survival.

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